Altered Affinity Maturation in Primary Response to (4-hydroxy-3-nitrophenyl) Acetyl (NP) after Autologous Reconstitution of Irradiated C57BL/6 Mice
| Autor: | Jacques Urbain, Annette Van Acker, Maryse Brait, Carl De Trez, Georgette Vansanten |
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| Přispěvatelé: | Cellular and Molecular Immunology, Department of Bio-engineering Sciences |
| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: |
lcsh:Immunologic diseases. Allergy
T-Lymphocytes Immunology Antibody Affinity Biology Antibodies Immunoglobulin G Nitrophenols Affinity maturation Spleen -- immunology -- radiation effects Mice Antigen Animals T-Lymphocytes -- immunology -- radiation effects Phenylacetates Lymphopenic environment Immunoglobulin G -- blood -- immunology B-Lymphocytes Germinal Center -- immunology -- radiation effects Germinal center Nitrophenols -- administration & dosage -- immunology Sciences bio-médicales et agricoles NP system Germinal Center Primary and secondary antibodies Molecular biology Antibody Affinity -- radiation effects Haptens -- immunology Mice Inbred C57BL Immunoglobulin class switching Radiation Chimera Antibodies -- blood -- immunology biology.protein B-Lymphocytes -- immunology -- radiation effects Immunization Germinal centers Irradiation Antibody lcsh:RC581-607 Haptens Hapten Spleen Research Article Developmental Biology |
| Zdroj: | Clinical and Developmental Immunology, Vol 9, Iss 3, Pp 119-125 (2002) Developmental Immunology, 9 (3 Vrije Universiteit Brussel Developmental Immunology |
| ISSN: | 1740-2530 1740-2522 |
| Popis: | Immune responses developing in irradiated environment are profoundly altered. The memory antiarsonate response of A/J mice is dominated by a major clonotype encoded by a single gene segment combination called CRIA. In irradiated and autoreconstituted A/J mice, the level of anti-ARS antibodies upon secondary immunization is normal but devoid of CRIA antibodies. The affinity maturation process and the somatic mutation frequency are reduced. Isotype switching and development of germinal centers (GC) are delayed. The primary antibody response of C57BL/6 mice to the hapten (4-hydroxy-3-nitrophenyl) acetyl (NP)-Keyhole Limpet Hemocyanin (KLH) is dominated by antibodies encoded by a family of closely related VH genes associated with the expression of the λ1 light chain. We investigated the anti-NP primary response in irradiated and autoreconstituted C57BL/6 mice. We observed some splenic alterations as previously described in the irradiated A/J model. Germinal center reaction is delayed although the extrafollicular foci appearance is unchanged. Irradiated C57BL/6 mice are able to mount a primary anti-NP response dominated by λ1 positive antibodies but fail to produce high affinity NP-binding IgG1 antibodies. Following a second antigenic challenge, irradiated mice develop enlarged GC and foci. Furthermore, higher affinity NP-binding IgG1 antibodies are detected. SCOPUS: cp.j info:eu-repo/semantics/published |
| Databáze: | OpenAIRE |
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