The central clock controls the daily rhythm of Aqp5 expression in salivary glands
| Autor: | Takahiro Nakamura, Aya Obana-Koshino, Takeshi Todo, Hitomi Ono, Hitoshi Uchida, Wataru Nakamura, Takayoshi Sakai, Nana N. Takasu |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Physiology Period (gene) Submandibular Gland Circadian clock Mice Transgenic Biology Mice 03 medical and health sciences 0302 clinical medicine Circadian Clocks Internal medicine medicine Animals Circadian rhythm Salivary gland Suprachiasmatic nucleus Period Circadian Proteins Aquaporin 5 Circadian Rhythm PER2 CLOCK 030104 developmental biology Endocrinology medicine.anatomical_structure Light effects on circadian rhythm Suprachiasmatic Nucleus 030217 neurology & neurosurgery |
| Zdroj: | The Journal of Physiological Sciences. 68:377-385 |
| ISSN: | 1880-6562 1880-6546 |
| DOI: | 10.1007/s12576-017-0540-1 |
| Popis: | Salivary secretion displays day-night variations that are controlled by the circadian clock. The central clock in the suprachiasmatic nucleus (SCN) regulates daily physiological rhythms by prompting peripheral oscillators to adjust to changing environments. Aquaporin 5 (Aqp5) is known to play a key role in salivary secretion, but the association between Aqp5 and the circadian rhythm is poorly understood. The aim of our study was to evaluate whether Aqp5 expression in submandibular glands (SMGs) is driven by the central clock in the SCN or by autonomous oscillations. We observed circadian oscillations in the activity of period circadian protein homolog 2 and luciferase fusion protein (PER2::LUC) in cultured SMGs with periodicity depending on core clock genes. A daily rhythm was detected in the expression profiles of Aqp5 in SMGs in vivo. In cultured SMGs ex vivo, clock genes showed distinct circadian rhythms, whereas Aqp5 expression did not. These data indicate that daily Aqp5 expression in the mouse SMG is driven by the central clock in the SCN. |
| Databáze: | OpenAIRE |
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