Association of Local Unit Sampling and Microbiology Laboratory Culture Practices With the Ability to Identify Causative Pathogens in Peritoneal Dialysis-Associated Peritonitis in Thailand

Autor: Surapong Narenpitak, Ampai Tongpliw, Puntapong Taruangsri, Nisa Thongbor, Apinya Wechpradit, Worapot Treamtrakanpon, Uraiwan Parinyasiri, Chadarat Kleebchaiyaphum, Korawee Sukmee, Chanchana Boonyakrai, Peerapach Rattanasoonton, Niparat Pikul, Rujira Luksanaprom, Talerngsak Kanjanabuch, Pichet Lorvinitnun, Phichit Songviriyavithaya, Setthapon Panyatong, Suchai Sritippayawan, Salakjit Pitakmongkol, Navarat Rukchart, Kanittha Triamamornwooth, Worauma Panya, Sirirat Sirinual, Sajja Tatiyanupanwong, Kittisak Tangjittrong, Bunpring Jaroenpattrawut, Nibondh Udomsantisuk, Narumon Lukrat, Pensri Uttayotha, Tanawin Nopsopon, Somchai Eiam-Ong, Ruchdaporn Phaichan, Wichai Sopassathit, Panthira Passorn, Anocha Pullboon, Nipa Aiyasanon, Siriwan Thaweekote, Angsuwarin Wongpiang, Niwat Lounseng, Tanittha Chatsuwan, Pimpong Wongtrakul, Sukit Nilvarangkul, Piyaporn Towannang, Yupha Laoong, Wandee Chantarungsri, Montha Jankramol, Jarubut Phisutrattanaporn, Guttiga Halue, Somphon Buranaosot, Warakoan Satitkan, Sriphrae Uppamai, Kearkiat Praditpornsilpa, Areewan Cheawchanwattana, Wanida Somboonsilp, Rutchanee Chieochanthanakij, Bruce G. Robinson, Metinee Chaiwut, Sayumporn Thaitrng, Thanchanok Buanet, Wadsamon Saikong, Wanlaya Thongsiw, Pongpratch Puapatanakul, David W. Johnson, Boontita Prasertkul, Donkum Kaewboonsert, Ussanee Poonvivatchaikarn, Kriang Tungsanga, Jeffrey Perl
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Kidney International Reports, Vol 6, Iss 4, Pp 1118-1129 (2021)
Kidney International Reports
ISSN: 2468-0249
Popis: Introduction This describes variations in facility peritoneal dialysis (PD) effluent (PDE) culture techniques and local microbiology laboratory practices, competencies, and quality assurance associated with peritonitis, with a specific emphasis on factors associated with culture-negative peritonitis (CNP). Methods Peritonitis data were prospectively collected from 22 Thai PD centers between May 2016 and October 2017 as part of the Peritoneal Dialysis Outcomes and Practice Patterns Study. The first cloudy PD bags from PD participants with suspected peritonitis were sent to local and central laboratories for comparison of pathogen identification. The associations between these characteristics and CNP were evaluated. Results CNP was significantly more frequent in local laboratories (38%) compared with paired PDE samples sent to the central laboratory (12%, P < 0.05). Marked variations were observed in PD center practices, particularly with respect to specimen collection and processing, which often deviated from International Society for Peritoneal Dialysis Guideline recommendations, and laboratory capacities, capabilities, and certification. Lower rates of CNP were associated with PD nurse specimen collection, centrifugation of PDE, immediate transfer of samples to the laboratory, larger hospital size, larger PD unit size, availability of an on-site nephrologist, higher laboratory capacity, and laboratory ability to perform aerobic cultures, undertake standard operating procedures in antimicrobial susceptibilities, and obtain local accreditation. Conclusion There were large variations in PD center and laboratory capacities, capabilities, and practices, which in turn were associated with the likelihood of culturing and correctly identifying organisms responsible for causing PD-associated peritonitis. Deviations in practice from International Society for Peritoneal Dialysis guideline recommendations were associated with higher CNP rates.
Graphical abstract
Databáze: OpenAIRE
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