Guard Cell-Specific Calcium Sensitivity of High Density and Activity SV/TPC1 Channels
| Autor: | Rainer Hedrich, Jörg Fromm, Florian Rienmüller, Edward E. Farmer, Irene Marten, Silke Lautner, Peter Ache, Khaled A. S. Al-Rasheid, Diana Beyhl |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Patch-Clamp Techniques
Physiology Mutant Arabidopsis High density Plant Science Biology Stress Physiological Guard cell Arabidopsis thaliana Gene Genetics Arabidopsis Proteins Sodium Plant physiology Conductance Cell Biology General Medicine biology.organism_classification Phenotype Cell biology Electrophysiological Phenomena Plant Leaves Arabidopsis/genetics Arabidopsis/metabolism Arabidopsis Proteins/genetics Arabidopsis Proteins/metabolism Calcium/metabolism Calcium Channels/genetics Calcium Channels/metabolism Electrophysiological Processes Mesophyll Cells/metabolism Mutation Plant Leaves/chemistry Plant Stomata/metabolism Potassium/analysis Sodium/analysis Vacuoles/metabolism Plant Stomata Vacuoles Potassium Calcium Calcium Channels Mesophyll Cells |
| Zdroj: | Plant and Cell Physiology, vol. 51, no. 9, pp. 1548-1554 |
| Popis: | The slow vacuolar (SV) channel, a Ca 2 + -regulated vacuolar cation conductance channel, in Arabidopsis thaliana is encoded by the single-copy gene AtTPC1 . Although loss-offunction tpc1 mutants were reported to exhibit a stoma phenotype, knowledge about the underlying guard cellspecifi c features of SV/TPC1 channels is still lacking. Here we demonstrate that TPC1 transcripts and SV current density in guard cells were much more pronounced than in mesophyll cells. Furthermore, the SV channel in motor cells exhibited a higher cytosolic Ca 2 + sensitivity than in mesophyll cells. These distinct features of the guard cell SV channel therefore probably account for the published stomatal phenotype of tpc1-2 . |
| Databáze: | OpenAIRE |
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