Differentiating Cerebellar Impact on Thalamic Nuclei
| Autor: | Freek E. Hoebeek, Oscar H J Eelkman Rooda, Alex L. Nigg, Simona V. Gornati, Chris I. De Zeeuw, Carmen B. Schäfer |
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| Přispěvatelé: | Netherlands Institute for Neuroscience (NIN), Neurosciences, Neurology, Neurosurgery, Pathology |
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Cerebellum Confocal Thalamus Stimulation cerebellar nuclei Optogenetics Neurotransmission Receptors Ionotropic Glutamate Article General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine thalamus morphology Journal Article medicine Animals synaptic transmission Axon optogenetics lcsh:QH301-705.5 Chemistry Excitatory Postsynaptic Potentials Dendrites Axons Electric Stimulation Mice Inbred C57BL Electrophysiology 030104 developmental biology medicine.anatomical_structure lcsh:Biology (General) nervous system Thalamic Nuclei Synapses Female Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Cell Reports [E], 23(9), 2690. Cell Press Cell Reports, Vol 23, Iss 9, Pp 2690-2704 (2018) Cell Reports, 23(9), 2690-2704. Cell Press Cell Reports |
| ISSN: | 2211-1247 |
| Popis: | Summary The cerebellum plays a role in coordination of movements and non-motor functions. Cerebellar nuclei (CN) axons connect to various parts of the thalamo-cortical network, but detailed information on the characteristics of cerebello-thalamic connections is lacking. Here, we assessed the cerebellar input to the ventrolateral (VL), ventromedial (VM), and centrolateral (CL) thalamus. Confocal and electron microscopy showed an increased density and size of CN axon terminals in VL compared to VM or CL. Electrophysiological recordings in vitro revealed that optogenetic CN stimulation resulted in enhanced charge transfer and action potential firing in VL neurons compared to VM or CL neurons, despite that the paired-pulse ratio was not significantly different. Together, these findings indicate that the impact of CN input onto neurons of different thalamic nuclei varies substantially, which highlights the possibility that cerebellar output differentially controls various parts of the thalamo-cortical network. Graphical Abstract Highlights • Cerebello-thalamic axons form terminals of varying size in distinct thalamic nuclei • Cerebello-thalamic responses vary in amplitude in distinct thalamic nuclei • Repetitive stimuli depress cerebello-thalamic responses in all thalamic nuclei In this study, Gornati et al. demonstrate that the impact of cerebellar nuclei axons on thalamic neurons varies per thalamic region. These findings provide insights into how the versatile cerebellum can have a differential effect on the many brain regions that it connects to. |
| Databáze: | OpenAIRE |
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