Standardization of acid hydrolysis procedure for urinary 3-methylhistidine determination by high-performance liquid chromatography
| Autor: | Roland N. Dickerson, David A. Kuhl, J T Methvin |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Chromatography
medicine.diagnostic_test Chemistry Proteolysis Hydrolysis Temperature General Chemistry Urine Hydrogen-Ion Concentration Methylhistidines High-performance liquid chromatography Rats Excretion Drug Stability medicine Animals Acid hydrolysis Thermal stability Quantitative analysis (chemistry) Chromatography High Pressure Liquid |
| Zdroj: | Journal of chromatography. B, Biomedical applications. 681(2) |
| ISSN: | 1572-6495 |
| Popis: | The N-acetylated form of N-methylhistidine (3-methylhistidine, 3-meH), a non-invasive marker of proteolysis, accounts for 80-90% of total 3-meH excretion (acetylated+non-acetylated 3-meH) in the rat. To determine total 3-meH excretion, samples require acid hydrolysis prior to determination by high-performance liquid chromatography. This study evaluated the stability of 3-meH at various times and temperatures of hydrolysis and determined the optimal conditions for hydrolysis of samples. Increasing temperature (120 degrees C) results in significant degradation of 3-meH with no appreciable change in concentration being noted at 80 degrees C. Hydrolysis at 100 degrees C for 1.5 to 4 h or 80 degrees C for 8 to 12 h is recommended for determining total 3-meH concentrations in rat urine. |
| Databáze: | OpenAIRE |
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