Efficient methanol-degrading aerobic bacteria isolated from a wetland ecosystem
| Autor: | Harikrishnan Kumarapillai, Aneesh Balakrishna Pillai, Kavitha Thulasi, Vinod Kumar Gopalakrishnapillai Sankaramangalam, Arjun Jayakumar |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Aerobic bacteria 030106 microbiology India Biochemistry Microbiology 03 medical and health sciences Bacterial Proteins Microbial ecology Botany Genetics Achromobacter spanius Molecular Biology Phylogeny Soil Microbiology biology Methanol dehydrogenase Chemistry Methanol General Medicine biology.organism_classification 16S ribosomal RNA Molecular Typing Alcohol Oxidoreductases Kinetics Biodegradation Environmental Methylobacterium 030104 developmental biology Methylobacterium radiotolerans Wetlands Methylotroph Bacteria |
| Zdroj: | Archives of Microbiology. 200:829-833 |
| ISSN: | 1432-072X 0302-8933 |
| DOI: | 10.1007/s00203-018-1509-z |
| Popis: | Methylotrophs present in the soil play an important role in the regulation of one carbon compounds in the environment, and thereby aid in mitigating global warming. The study envisages the isolation and characterization of methanol-degrading bacteria from Kuttanad wetland ecosystem, India. Three methylotrophs, viz. Achromobacter spanius KUT14, Acinetobacter sp. KUT26 and Methylobacterium radiotolerans KUT39 were isolated and their phylogenetic positions were determined by constructing a phylogenetic tree based on 16S rDNA sequences. In vitro activity of methanol dehydrogenase enzyme, responsible for methanol oxidation was evaluated and the genes involved in methanol metabolism, mxaF and xoxF were partially amplified and sequenced. The specific activity of methanol dehydrogenase (451.9 nmol min−1 mg−1) observed in KUT39 is the highest, reported ever to our knowledge from a soil bacterium. KUT14 recorded the least activity of 50.15 nmol min−1 mg−1 and is the first report on methylotrophy in A. spanius. |
| Databáze: | OpenAIRE |
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