Evaluation and optimization of differentiation conditions for human primary brown adipocytes
Autor: | Lianghui You, Xing Wang, Xianwei Cui, Pengfei Xu, Yun Li, Xingyun Wang, Lijun Zhu, Juan Wen, Lingxia Pang, Chenbo Ji, Xirong Guo |
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Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Brown Adipocytes Cell Culture Techniques Adipose tissue lcsh:Medicine Biology Article 03 medical and health sciences Fetus Adipose Tissue Brown Brown adipose tissue Gene expression medicine Humans Obesity lcsh:Science Cells Cultured Adipogenesis Multidisciplinary Triiodothyronine lcsh:R Cell Differentiation Thermogenesis White adipocyte differentiation Cell biology Adipocytes Brown 030104 developmental biology medicine.anatomical_structure Energy expenditure lcsh:Q Energy Metabolism |
Zdroj: | Scientific Reports, Vol 8, Iss 1, Pp 1-12 (2018) Scientific Reports |
ISSN: | 2045-2322 |
Popis: | As an effective way to improve energy expenditure, increasing the mass and activity of brown adipose tissue (BAT) has become a promising treatment for obesity and its associated disorders. Many efforts have been made to promote brown adipogenesis and increase the thermogenic capacity of brown adipose cells (BACs). The present culture schemes for human BAC differentiation are mostly derived from white adipocyte differentiation schemes. To solve this issue, we compared the adipogenic and thermogenic effects of various components on human BAC differentiation and optimized their concentrations as well as the culture time for BAC differentiation. In this study, we found that the induction factors did not show a dose-dependent promotion of brown adipogenesis or thermogenic capacity. The higher differentiation levels did not inevitably result in higher BAT-specific gene expression levels or increased β3-receptor agonist sensitivity. As an important element of culture medium, triiodothyronine was found to be essential for differentiation and metabolic property maintenance. Furthermore, compared with other reported methods, this protocol induced a specific intrinsic differentiation program. Our study provides not only an optimized method for human BAC differentiation but also a cell model with good differentiation and thermogenic capacity for brown adipose research. |
Databáze: | OpenAIRE |
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