Gold Nanoparticles-Amplified Indirect Competitive Enzyme-Linked Immunosorbent Assay of BDE-121 Using a Monoclonal Antibody
| Autor: | Qing-Yun Cai, Yan Qiao |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Materials science
medicine.drug_class Biomedical Engineering Metal Nanoparticles Enzyme-Linked Immunosorbent Assay Bioengineering 02 engineering and technology 010501 environmental sciences Mass spectrometry Monoclonal antibody 01 natural sciences Halogenated Diphenyl Ethers medicine General Materials Science IC50 0105 earth and related environmental sciences chemistry.chemical_classification Chromatography Antibodies Monoclonal General Chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics Enzyme chemistry Colloidal gold Gold Gas chromatography 0210 nano-technology Hapten |
| Zdroj: | Journal of Nanoscience and Nanotechnology. 21:5036-5043 |
| ISSN: | 1533-4880 |
| Popis: | In this study, we developed a monoclonal antibody against 2,3’,4,5’,6-pentabromodiphenylether (BDE-121) using a synthesized hapten, and established an indirect competitive enzyme-linked immunosorbent assay (IC-ELISA), using gold nanoparticles, to amplify the signal. The monoclonal antibody showed high specificity, with a half inhibitory concentration (IC50) value of 2.78 ng/mL, towards BDE-121. The developed IC-ELISA exhibited high sensitivity and stability as well as good recovery. The intra-assay deviation is below 6.8% and the inter-assay deviations range from 6.5% to 8.7%. The assay of the actual samples was found to be consistent with those of gas chromatography/mass spectrometry (GC/MS). |
| Databáze: | OpenAIRE |
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