Effects of Radiofrequency Electromagnetic Fields and Ionizing Radiation on Amyloid Precursor Protein Processing and Cell Death
| Autor: | Sang Bong Jeon, Kyung Min Lim, Yun Sil Lee, Hae June Lee, Sojung Choi, Hyung-Do Choi, Nam Kim, Kyeonghee Yoon |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Electromagnetic field Programmed cell death QC501-766 Computer Networks and Communications amyloid protein precursor processing Ionizing radiation 03 medical and health sciences 0302 clinical medicine Amyloid precursor protein Medicine Electrical and Electronic Engineering Instrumentation Radiation biology business.industry TK1-9971 Electricity and magnetism 030104 developmental biology cell death biology.protein Biophysics Electrical engineering. Electronics. Nuclear engineering business ionizing radiation rf-emf 030217 neurology & neurosurgery |
| Zdroj: | Journal of Electromagnetic Engineering and Science, Vol 20, Iss 4, Pp 307-319 (2020) |
| ISSN: | 2671-7263 2671-7255 |
| Popis: | The growing concerns regarding the adverse biological effects of radiofrequency electromagnetic fields (RF-EMFs), which are generated by common electronic devices, on the human brain led us to investigate their impact on Alzheimer’s disease (AD). We aimed to establish the effects of RF-EMF on the expression of molecular markers associated with amyloid precursor protein (APP), cell death, and clonogenic survival in HT22 and APP-overexpressing 7w-PSML cells. We compared the effects of RF-EMF at a high specific absorption rate (SAR) level with the neuronal-cell-death-inducing effects of ionizing radiation (IR). RF-EMF exposure (8 W/kg SAR) promoted the protein expression of ADAM10 (α-secretase) in the HT22 cells (p < 0.05) and downregulated the APP mRNA level in the 7w-PSML cells (p < 0.01). In contrast, IR (10 Gy) significantly reduced the APP and a disintegrin and metalloproteinase 10 (ADAM10) levels without altering their respective mRNA levels in these cells. Interestingly, IR exposure significantly upregulated BACE1 (α-secretase) at both the protein and mRNA levels, suggesting adverse effects in AD. IR induced cell death and reduced clonogenic survival in both cell lines. Although RF-EMF (high SAR level) influenced APP processing, it did not induce any deleterious change in either cell line. Thus, further studies are necessary to clarify the influence of RF-EMF on AD. |
| Databáze: | OpenAIRE |
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