Decreased VEGF concentration in lung tissue and vascular injury during ARDS
| Autor: | Serge Adnot, Laurent Papazian, Fabienne Brégeon, Y. Abadie, Christophe Delclaux, Bernard Maitre, F. Lange, Bernadette Chailley-Heu, P. Duvaldestin, Pascal Thomas |
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| Rok vydání: | 2005 |
| Předmět: |
Male
Vascular Endothelial Growth Factor A Pulmonary and Respiratory Medicine CD31 Pathology medicine.medical_specialty ARDS Endothelium Sensitivity and Specificity Severity of Illness Index Cohort Studies chemistry.chemical_compound Reference Values Risk Factors Humans Medicine Vascular Diseases Aged Respiratory Distress Syndrome Lung business.industry Biopsy Needle Respiratory disease Middle Aged Prognosis medicine.disease Immunohistochemistry Survival Rate Vascular endothelial growth factor Endothelial stem cell medicine.anatomical_structure chemistry Case-Control Studies Female Endothelium Vascular business Biomarkers Immunostaining |
| Zdroj: | European Respiratory Journal. 25:139-146 |
| ISSN: | 1399-3003 0903-1936 |
| DOI: | 10.1183/09031936.04.00065504 |
| Popis: | Endothelial injury is an important prognostic factor in acute respiratory distress syndrome (ARDS). Decreased production of vascular endothelial growth factor (VEGF) in ARDS may favour vascular lesions, since VEGF promotes endothelial survival by inhibiting apoptosis. This study sought to document low VEGF levels in lung tissue from ARDS patients, to determine whether the cause was injury to alveolar type II cells (the main pulmonary source of VEGF) and to evaluate the vascular consequences. Lung specimens were obtained by open biopsy or autopsy from 29 patients with severe ARDS (two survivors) and five controls. As compared with controls, homogenates of lung tissue from ARDS patients contained less VEGF (median (interquartile range) ARDS 8.2 (4.7-12.2) versus controls 28.4 (9.9-47.1) ng x g(-1) protein). Increased immunostaining with surfactant protein B was seen in ARDS lungs. Extensive cellular apoptosis (terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labelling staining), including endothelial and alveolar type II cells, was demonstrated, and vascular bed density (CD31 immunostaining) decreased in ARDS lungs as compared with controls. VEGF levels were negatively correlated to apoptotic endothelial cell counts. In conclusion, decreased vascular endothelial growth factor levels in lung tissue may participate in the decrease in lung perfusion in acute respiratory distress syndrome. |
| Databáze: | OpenAIRE |
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