Tensiometric and Phase Domain Behavior of Lung Surfactant on Mucus-like Viscoelastic Hydrogels
| Autor: | Jennifer Fiegel, Daniel Schenck |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Calfactant Materials science Acrylic Resins 02 engineering and technology Viscoelasticity Article Surface tension 03 medical and health sciences Rheology Pulmonary surfactant Phase (matter) Elastic Modulus medicine Animals Surface Tension General Materials Science Lung Biological Products Chromatography Viscosity Hydrogels Pulmonary Surfactants respiratory system 021001 nanoscience & nanotechnology Mucus 030104 developmental biology Interferometry Chemical engineering Microscopy Fluorescence Self-healing hydrogels Cattle 0210 nano-technology medicine.drug |
| Zdroj: | ACS applied materialsinterfaces. 8(9) |
| ISSN: | 1944-8252 |
| Popis: | Lung surfactant has been observed at all surfaces of the airway lining fluids and is an important contributor to normal lung function. In the conducting airways, the surfactant film lies atop a viscoelastic mucus gel. In this work, we report on the characterization of the tensiometric and phase domain behavior of lung surfactant at the air-liquid interface of mucus-like viscoelastic gels. Poly(acrylic acid) hydrogels were formulated to serve as a model mucus with bulk rheological properties that matched those of tracheobronchial mucus secretions. Infasurf® (Calfactant), a commercially available pulmonary surfactant derived from calf lung extract, was spread onto the hydrogel surface. The surface tension lowering ability and relaxation of Infasurf films on the hydrogels was quantified and compared to Infasurf behavior on an aqueous subphase. Infasurf phase domains during surface compression were characterized by fluorescence microscopy and phase shifting interferometry. We observed that increasing the bulk viscoelastic properties of the model mucus hydrogels reduced the ability of Infasurf films to lower surface tension and inhibited film relaxation. A shift in the formation of Infasurf condensed phase domains from smaller, more spherical domains to large, agglomerated, multilayer structures was observed with increasing viscoelastic properties of the subphase. These studies demonstrate that the surface behavior of lung surfactant on viscoelastic surfaces, such as those found in the conducting airways, differs significantly from aqueous, surfactant-laden systems. |
| Databáze: | OpenAIRE |
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