Percutaneous Penetration of Three Insecticides in Rats: A Comparison of Two Methods for In Vivo Determination
| Autor: | Prakashchandra V. Shah, F. E. Guthrie |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Male
Insecticides Swine Dermatology Urine Pharmacology Carbaryl Biochemistry DDT Excretion chemistry.chemical_compound In vivo Methods Animals Humans Saimiri Molecular Biology Feces Skin Chromatography Parathion Rats Inbred Strains Cell Biology Penetration (firestop) Rats Skin patch Liver chemistry Rabbits |
| Zdroj: | Journal of Investigative Dermatology. 80:291-293 |
| ISSN: | 0022-202X |
| DOI: | 10.1111/1523-1747.ep12534663 |
| Popis: | Percutaneous penetration of three insecticides was studied by two methods. The indirect (excretion analysis) and direct (skin patch removal) methods for determining penetration were compared in rats. Radiolabeled solutions of parathion, carbaryl, and DDT were applied to previously shaved rats at the rate of 4 micrograms/cm2. Recoveries of radioactivity in urine, feces, application site, and various tissues were measured at intervals over a 5-day period. Urinary excretion rates were corrected for incomplete excretion by intraperitoneal applications. In the 5 days following intraperitoneal administration, the urinary excretion of parathion and carbaryl was greater than 80% while less than 5% of DDT was excreted. A good correlation was found between the indirect and direct methods utilized to determine percutaneous absorption rates with the compounds tested at the later time intervals. All compounds showed more than 85% dermal penetration within 5 days. At the early time intervals (greater than 24 h), penetration by the direct method was significantly greater for parathion and carbaryl than by the indirect method. |
| Databáze: | OpenAIRE |
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