An integrated host-microbiome response to atrazine exposure mediates toxicity in Drosophila
| Autor: | Jian-Hua Mao, Siti Nur Sarah Morris, Susan E. Celniker, James B. Brown, William W. Fisher, Kenneth H. Wan, Richard Weiszmann, Mark R. Viant, Benjamin W. Booth, Charles Yu, Soo Park, Ann S. Hammonds, Jennifer A. Kirwan, Sasha A. Langley, Antoine M. Snijders, Ralf J. M. Weber |
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| Rok vydání: | 2021 |
| Předmět: |
Male
Insecticides QH301-705.5 Phenotypic screening Medicine (miscellaneous) Biology General Biochemistry Genetics and Molecular Biology Article Inactivation chemistry.chemical_compound Immune system Detoxification Genetics Metabolomics Animals Acetobacter 2.1 Biological and endogenous factors 2.2 Factors relating to the physical environment Microbiome Atrazine Biology (General) Aetiology Transcriptomics Drosophila Nutrition Host Microbial Interactions Host (biology) biology.organism_classification Gastrointestinal Microbiome Drosophila melanogaster chemistry Inactivation Metabolic Female Metabolic General Agricultural and Biological Sciences |
| Zdroj: | Communications biology, vol 4, iss 1 Communications Biology Communications Biology, Vol 4, Iss 1, Pp 1-12 (2021) |
| Popis: | The gut microbiome produces vitamins, nutrients, and neurotransmitters, and helps to modulate the host immune system—and also plays a major role in the metabolism of many exogenous compounds, including drugs and chemical toxicants. However, the extent to which specific microbial species or communities modulate hazard upon exposure to chemicals remains largely opaque. Focusing on the effects of collateral dietary exposure to the widely used herbicide atrazine, we applied integrated omics and phenotypic screening to assess the role of the gut microbiome in modulating host resilience in Drosophila melanogaster. Transcriptional and metabolic responses to these compounds are sex-specific and depend strongly on the presence of the commensal microbiome. Sequencing the genomes of all abundant microbes in the fly gut revealed an enzymatic pathway responsible for atrazine detoxification unique to Acetobacter tropicalis. We find that Acetobacter tropicalis alone, in gnotobiotic animals, is sufficient to rescue increased atrazine toxicity to wild-type, conventionally reared levels. This work points toward the derivation of biotic strategies to improve host resilience to environmental chemical exposures, and illustrates the power of integrative omics to identify pathways responsible for adverse health outcomes. Brown et al. apply integrated omics and phenotypic screening to assess the role of the gut microbiome in modulating host resilience in Drosophila melanogaster. They find that Acetobacter tropicalis in gnotobiotic animals, is sufficient to rescue increased atrazine toxicity, which could pave the way for biotic strategies to improve host resilience to environmental chemical exposure. |
| Databáze: | OpenAIRE |
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