Spin label EPR suggests the presence of cholesterol rich domains in cultured insect cell membranes
| Autor: | Verónica Viviana Gioria, Nieves M. C. Casado, Gabriela A. Micheloud, Maria Alejandra Baque, Ana Maria Gennaro, Juan Daniel Claus |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Biophysics CHOLESTANE SPIN LABEL Biochemistry law.invention Ciencias Biológicas 03 medical and health sciences chemistry.chemical_compound CULTURED INSECT CELLS law MEMBRANE FLUIDITY Membrane fluidity Animals Spin (physics) Spin label Electron paramagnetic resonance Molecular Biology Lipid raft Cells Cultured Insect cell Chemistry Cholesterol CHOLESTEROL Cell Membrane Electron Spin Resonance Spectroscopy Cell Biology Lipids Biofísica Lepidoptera MEMBRANE NANODOMAINS LIPID RAFTS 030104 developmental biology Membrane Liposomes Spin Labels CIENCIAS NATURALES Y EXACTAS |
| Popis: | Different spin labels were incorporated to the membranes of cultured insect UFL-AG-286 cells in order to characterize their physical properties by Electron Paramagnetic Resonance spectroscopy (EPR). The spectrum of the spin label 12-SASL incorporated to cell membranes was similar as those obtained in membrane model systems composed of eggPC/cholesterol. However, the spectrum of the spin label CSL, chemically related to cholesterol, was drastically different in the two systems. Interestingly, when cell cholesterol content was reduced using methyl beta cyclodextrin, an EPR spectrum similar to those of model membranes was obtained. The analysis of these experiments suggests the existence of cholesterol rich regions in UFL-AG-286 cell membranes. Fil: Baque, Maria Alejandra. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Física; Argentina Fil: Gioria, Verónica Viviana. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Virologia; Argentina Fil: Micheloud, Gabriela Analia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina Fil: Casado, Nieves Maria Claudia. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Física; Argentina Fil: Claus, Juan Daniel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Virologia; Argentina Fil: Gennaro, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Física del Litoral. Universidad Nacional del Litoral. Instituto de Física del Litoral; Argentina |
| Databáze: | OpenAIRE |
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