Type 1 Diabetes Impairs Cardiomyocyte Contractility in the Left and Right Ventricular Free Walls but Preserves It in the Interventricular Septum

Autor: Anastasia Khokhlova, Tatiana Myachina, Denis Volzhaninov, Xenia Butova, Anastasia Kochurova, Valentina Berg, Irina Gette, Gleb Moroz, Svetlana Klinova, Ilzira Minigalieva, Olga Solovyova, Irina Danilova, Ksenia Sokolova, Galina Kopylova, Daniil Shchepkin
Rok vydání: 2021
Předmět:
Male
endocrine system diseases
single cardiomyocyte mechanics
VENTRICULAR FUNCTION
CALCIUM TRANSIENTS
HEART CONTRACTION
diabetic cardiomyopathy
Ventricular Function
Myocytes
Cardiac
Biology (General)
Spectroscopy
ACTIN-MYOSIN INTERACTION
General Medicine
WISTAR RAT
interventricular differences
calcium transients
tension-length relationship
actin-myosin interaction
Computer Science Applications
INSULIN DEPENDENT DIABETES MELLITUS
Chemistry
TENSION-LENGTH RELATIONSHIP
RATS
WISTAR
QH301-705.5
macromolecular substances
METABOLISM
Catalysis
CALCIUM
RATS
Inorganic Chemistry
INTERVENTRICULAR DIFFERENCES
CARDIAC MUSCLE CELL
DIABETIC CARDIOMYOPATHY
MYOCARDIAL CONTRACTION
MYOCYTES
CARDIAC
Animals
Physical and Theoretical Chemistry
Rats
Wistar
Molecular Biology
QD1-999
SINGLE CARDIOMYOCYTE MECHANICS
MALE
Organic Chemistry
ANIMALS
ANIMAL
HEART VENTRICLE FUNCTION
Myocardial Contraction
Rats
PATHOLOGY
Diabetes Mellitus
Type 1
DIABETES MELLITUS
TYPE 1
RAT
Calcium
Zdroj: International Journal of Molecular Sciences; Volume 23; Issue 3; Pages: 1719
International Journal of Molecular Sciences, Vol 23, Iss 1719, p 1719 (2022)
Int. J. Mol. Sci.
International Journal of Molecular Sciences
ISSN: 1422-0067
Popis: Type 1 diabetes (T1D) leads to ischemic heart disease and diabetic cardiomyopathy. We tested the hypothesis that T1D differently affects the contractile function of the left and right ventricular free walls (LV, RV) and the interventricular septum (IS) using a rat model of alloxan-induced T1D. Single-myocyte mechanics and cytosolic Ca2+ concentration transients were studied on cardiomyocytes (CM) from LV, RV, and IS in the absence and presence of mechanical load. In addition, we analyzed the phosphorylation level of sarcomeric proteins and the characteristics of the actin-myosin interaction. T1D similarly affected the characteristics of actin-myosin interaction in all studied regions, decreasing the sliding velocity of native thin filaments over myosin in an in vitro motility assay and its Ca2+ sensitivity. A decrease in the thin-filament velocity was associated with increased expression of β-myosin heavy-chain isoform. However, changes in the mechanical function of single ventricular CM induced by T1D were different. T1D depressed the contractility of CM from LV and RV; it decreased the auxotonic tension amplitude and the slope of the active tension-length relationship. Nevertheless, the contractile function of CM from IS was principally preserved. © 2022 by the authors. Licensee MDPI, Basel, Switzerland. Funding: This work was supported by the Russian Science Foundation № 18-74-10059. The work was performed using the equipment of the Shared Research Center of Scientific Equipment of Institute of Immunology and Physiology.
Databáze: OpenAIRE
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