The role of cyclooxygenase-1 and cyclooxygenase-2 in lipopolysaccharide and interleukin-1 stimulated enterocyte prostanoid formation

Autor:	Ninder Panesar, John E. Mazuski, Donald L. Kaminski, L J Damore, Gregory S. Smith, Walter E. Longo
Rok vydání:	1998
Předmět:	Lipopolysaccharides medicine.medical_specialty Leukotriene B4 Prostaglandin E2 receptor Immunology Prostaglandin Prostacyclin Dinoprostone chemistry.chemical_compound Ileum Internal medicine Inhibitors Discovery and development of cyclooxygenase 2 inhibitors lcsh:Pathology medicine Animals Prostaglandin E2 Cells Cultured biology Prostaglandin D2 Prostaglandins F Membrane Proteins Cell Biology Cyclooxygenase Rats Isoenzymes Endocrinology chemistry Cyclooxygenase 2 Prostaglandin-Endoperoxide Synthases Cyclooxygenase 1 Prostaglandins biology.protein Cytokines Eicosanoids lipids (amino acids peptides and proteins) Mitogens lcsh:RB1-214 Research Article Interleukin-1 medicine.drug
Zdroj:	Mediators of Inflammation Mediators of Inflammation, Vol 7, Iss 2, Pp 85-91 (1998)
ISSN:	1466-1861 0962-9351
DOI:	10.1080/09629359891225
Popis:	Lipopolysaccharide is an inflammatory agent and interleukin-1 is a cytokine. Their pro-inflammatory effects may be mediated by prostanoids produced by inducible cyclooxygenase-2. The aim of this study was to determine the prostanoids produced by lipopolysaccharide and interleukin-1 stimulated enterocytes through the cyclooxygenase-1 and 2 pathways. Cultured enterocytes were stimulated with lipopolysaccharide or interleukin-1 β with and without cyclooxygenase inhibitors. Low concentrations of indomethacin and valerylsalicylic acid (VSA) were evaluated as cyclooxygenase-1 inhibitors and their effects compared with the effects of a specific cyclooxygenase-2 inhibitor, SC-58125. Prostaglandin E2, 6-keto prostaglandin F1α, prostaglandin D2and leukotriene B4levels were determined by radio immunoassay. Immunoblot analysis using isoformspecific antibodies showed that the inducible cyclooxygenase enzyme (COX-2) was expressed by 4 h in LPS and IL-1β treated cells while the constitutive COX-1 remained unaltered in its expression. Interleukin-1β and lipopolysaccharide stimulated the formation of all prostanoids compared with untreated cells, but failed to stimulate leukotriene B4. Indomethacin at 20 μ M concentration, and VSA inhibited lipopolysaccharide and interleukin 1β stimulated prostaglandin E2, but not 6-keto prostaglandin F1αformation. SC-58125 inhibited lipopolysaccharide and interleukin-1β stimulated 6-keto prostaglandin F1αbut not prostaglandin E2release. The specific cyclooxygenase-2 inhibitor also inhibited lipopolysaccharide produced prostaglandin D2but not interleukin-1β stimulated prostaglandin D2While SC-58125 inhibited basal 6-keto prostaglandin-F1αformation it significantly increased basal prostaglandin E2and prostaglandin D2formation. As SC-58125 inhibited lipopolysaccharide and interleukin-1β induced 6-keto prostaglandin F1αproduction but not prostaglandin E2production, it suggests that these agents stimulate prostacyclin production through a cyclooxygenase-2 mediated mechanism and prostaglandin E2production occurs through a cyclooxygenase-1 mediated mechanism. Prostaglandin D2production appeared to be variably produced by cyclooxygenase-1 or cyclooxygenase-2, depending on the stimulus.
Databáze:	OpenAIRE
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