Dried blood spot specimen quality and validation of a new pre-analytical processing method for qualitative HIV-1 PCR, KwaZulu-Natal, South Africa
| Autor: | Ntombizandile Siyaca, Raveen Parboosing, Pravikrishnen Moodley, Kerusha Govender |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Clinical Biochemistry Dried blood spot specimen Human immunodeficiency virus (HIV) Dentistry Not provided medicine.disease_cause 03 medical and health sciences 0302 clinical medicine Medicine 030212 general & internal medicine Original Research business.industry Pre analytical lcsh:Public aspects of medicine Public Health Environmental and Occupational Health lcsh:RA1-1270 030112 virology Dried blood spot Processing methods Medical Laboratory Technology Specimen Quality Specimen volume business Kwazulu natal |
| Zdroj: | African Journal of Laboratory Medicine, Vol 5, Iss 1, Pp e1-e6 (2016) African Journal of Laboratory Medicine African Journal of Laboratory Medicine, Volume: 5, Issue: 1, Pages: 1-6, Published: 2016 |
| ISSN: | 2225-2010 2225-2002 |
| Popis: | Background: Poor quality dried blood spot (DBS) specimens are usually rejected by virology laboratories, affecting early infant diagnosis of HIV. The practice of combining two incompletely-filled DBS in one specimen preparation tube during pre-analytical specimen processing (i.e., the two-spot method) has been implemented to reduce the number of specimens being rejected for insufficient volume.Objectives: This study analysed laboratory data to describe the quality of DBS specimens and the use of the two-spot method over a one-year period, then validated the two-spot method against the standard (one-spot) method.Methods: Data on HIV-1 PCR test requests submitted in 2014 to the Department of Virology at Inkosi Albert Luthuli Central Hospital in KwaZulu-Natal province, South Africa were analysed to describe reasons for specimen rejection, as well as results of the two-spot method. The accuracy, lower limit of detection and precision of the two-spot method were assessed.Results: Of the 88 481 specimens received, 3.7% were rejected for pre-analytical problems. Of those, 48.9% were rejected as a result of insufficient specimen volume. Two health facilities had significantly more specimen rejections than other facilities. The two-spot method prevented 10 504 specimen rejections. The Pearson correlation coefficient comparing the standard to the two-spot method was 0.997.Conclusions: The two-spot method was comparable with the standard method of pre-analytical specimen processing. Two health facilities were identified for targeted retraining on specimen quality. The two-spot method of DBS specimen processing can be used as an adjunct to retraining, to reduce the number of specimens rejected and improve linkage to care. |
| Databáze: | OpenAIRE |
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