Development and characterization of microsatellite markers for the oomyceta [i]Aphanomyces euteiches[/i]
| Autor: | M.L. Pilet, A. Quillévéré, Lucie Mieuzet, C. Le May |
|---|---|
| Přispěvatelé: | Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, The authors thank the AAP 'Gestion Durable des Résistances' (GDR) program (Project GestAphaleg) for funding. This funding was delivered by SPE ('Santé des Plantes et Environnement'), BAP ('Biologie et Amélioration des Plantes'), EA ('Environnement et Agronomie'), EF ('Ecologie des Forêts'), PmA ('Prairies et milieu Aquatiques ») INRA departments. Authors would like to thanks A. Moussart and M.N. Even (Terres Inovia, UMT PISOM) for their valuable help in collecting soils from the different locations., Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Population Population genetics Aphanomyces Locus (genetics) 01 natural sciences Microbiology Plant Roots Genetic study 03 medical and health sciences Genetics Root rot [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Genomic library education Oomyceta Alleles Plant Diseases 2. Zero hunger education.field_of_study biology Peas Chromosome Mapping food and beverages Microsatellite biology.organism_classification SSR 030104 developmental biology Aphanomyces euteiches 010606 plant biology & botany Microsatellite Repeats |
| Zdroj: | Fungal Genetics and Biology Fungal Genetics and Biology, Elsevier, 2016, 91, pp.1-5. ⟨10.1016/j.fgb.2016.03.001⟩ Fungal Genetics and Biology, 2016, 91, pp.1-5. ⟨10.1016/j.fgb.2016.03.001⟩ |
| ISSN: | 1087-1845 1096-0937 |
| Popis: | International audience; Aphanomyces euteiches Drechsler is a serious pathogen of leguminous crops that causes devastating root rot of pea worldwide. Given that A. euteiches is a diploid organism, robust, codominant markers are needed for population genetics studies. We have developed and screened a microsatellite-enriched small-insert genomic library for identification of A. euteiches SSR containing sequences. Fourteen out of the 48 primer pairs designed to amplify SSR, produced unambiguous polymorphic products in our test population of 94 isolates. The number of alleles at each locus ranged from one to four. The identification of new markers would enhance the ability to evaluate the genetic structure of A. euteiches populations, and pathogen evolution. |
| Databáze: | OpenAIRE |
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