Analysis of Biogenic Amines Using Immunoassays, HPLC, and a Newly Developed IC-MS/MS Technique in Fish Products—A Comparative Study
| Autor: | Serkan Koral, Matevž Pompe, Andrej Ščavničar, Bekir Tufan, Sevim Köse, Drago Kočar |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Ion chromatography
Pharmaceutical Science Organic chemistry biogenic amines High-performance liquid chromatography Article Analytical Chemistry chemistry.chemical_compound QD241-441 IC-MS/MS Tandem Mass Spectrometry Drug Discovery medicine fish products Physical and Theoretical Chemistry histamin Derivatization udc:543.544:547.233 biogeni amini imunotest Chromatography High Pressure Liquid Immunoassay Cadaverine ribji produkti Chromatography medicine.diagnostic_test Tyramine Fish products Chromatography Ion Exchange histamine immunoassays chemistry Chemistry (miscellaneous) method comparison Molecular Medicine primerjava metod HPLC Histamine |
| Zdroj: | Molecules, Vol 26, Iss 6156, p 6156 (2021) Molecules Molecules, vol. 26, no. 20, 6156, 2021. Volume 26 Issue 20 |
| ISSN: | 1420-3049 |
| Popis: | In this study, comparative analyses were carried out with ion chromatography mass-spectrometry (IC-MS/MS) which has no derivatization step, high-performance liquid chromatography (HPLC) technique, as well as two quantitative and two semi-quantitative immunoassays. The results demonstrated that HPLC and quantitative immunoassay methods were well-correlated with IC-MS/MS in determining histamine in various types of fish products. The best correlation was observed with the HistaSure ELISA Fast Track kit (R2 = 0.9903). More than half of the values (68%) obtained by two methods were also statistically similar. The results of semi-quantitative test kits also supported histamine values estimated by quantitative methods, with some exceptions. The best results were found for HistaSure Lateral Flow in supporting the quantitative techniques. Therefore, these methods are found suitable for monitoring histamine in fish products in terms of food safety. Good correlations were also observed HPLC and IC-MS/MS in determining cadaverine, putrescine, and tyramine with the highest value observed for tyramine as R2 = 0.9785. However, no correlation was observed for other biogenic amines, and the majority of the results were significantly different from each other for these amines (p < 0.05). The differences may be caused by the drawbacks reported previously for HPLC. However, further studies are required to confirm the possible effects. This study provides a comparative evaluation of several methods in terms of their suitability in determining biogenic amines in fish products for both monitoring and regulatory purposes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|