Analysis of pectin from Panax ginseng flower buds and their binding activities to galectin-3
| Autor: | Ya Tan, Liangnan Cui, Jiayi Wang, Rui Huang, Fan Zhang, Yifa Zhou, Lin Sun |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
food.ingredient
Pectin Galectin 3 Size-exclusion chromatography Panax 02 engineering and technology Flowers Polysaccharide Biochemistry 03 medical and health sciences Ginseng food Structural Biology Arabinogalactan Enzymatic hydrolysis Side chain Molecular Biology 030304 developmental biology chemistry.chemical_classification 0303 health sciences Chemistry General Medicine 021001 nanoscience & nanotechnology Covalent bond Pectins 0210 nano-technology Protein Binding |
| Zdroj: | International journal of biological macromolecules. 128 |
| ISSN: | 1879-0003 |
| Popis: | Water-soluble pectic polysaccharides isolated from Panax ginseng flower buds (WGFPA) were completely fractionated into six homogeneous fractions (WGFPA-1a, WGFPA-2a, WGFPA-3a, WGFPA-1b, WGFPA-2b and WGFPA-3b) by a combination of ion-exchange and size exclusion chromatographies. Monosaccharide composition, enzymatic hydrolysis and 13C nuclear magnetic resonance (NMR) spectra analysis were combined to characterize their structural features. Furthermore, the interactions between these polysaccharides and galectin-3 were evaluated by biolayer interferometry assay. The results showed that WGFPA-1a, WGFPA-2a and WGFPA-3a were rhamnogalacturonan I (RG-I) type pectin with abundant side chains, including α-L-1,5-arabinan, β-D-1,4-galactan, arabinogalactan I (AG-I) and arabinogalactan II (AG-II), exhibiting strong binding activities to galectin-3 with apparent KD values 4.9 μM, 0.71 μM and 0.24 μM, respectively. WGFPA-1b, WGFPA-2b and WGFPA-3b were homogalacturonan (HG) type pectin covalently linked with different ratios of rhamnogalacturonan II (RG-II) domains, showing weaker or no interactions with galectin-3. This study provides useful structural information for further investigation on the structure-activity relationship of ginseng flower buds pectin. |
| Databáze: | OpenAIRE |
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