Dysbiosis of gut microbiome affecting small intestine morphology and immune balance: a rhesus macaque model
| Autor: | Hongzhe Li, Nan Li, Haitao Fan, Xing Huang, Manman Chu, Huiwen Zheng, Jinxi Yang, Yuan Zhao, Qiongwen Wu, Lei Guo, Zhanlong He, Zening Yang, Jingjing Wang, Longding Liu, Heng Li |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
DNA Bacterial Male Biology Peripheral blood mononuclear cell Microbiology Transcriptome 03 medical and health sciences Feces 0302 clinical medicine Immune system Pathological changes lcsh:Zoology Rhesus macaque medicine Animals lcsh:QL1-991 Microbiome KEGG Immune response Ecology Evolution Behavior and Systematics Gut microbiome Ecology Bacteria Articles medicine.disease biology.organism_classification Macaca mulatta Antibiotic treatment Small intestine Anti-Bacterial Agents Gastrointestinal Microbiome Intestines 030104 developmental biology medicine.anatomical_structure Dysbiosis Animal Science and Zoology 030217 neurology & neurosurgery |
| Zdroj: | Zoological Research Zoological Research, Vol 41, Iss 1, Pp 20-31 (2020) |
| ISSN: | 2095-8137 |
| Popis: | There is a growing appreciation for the specific health benefits conferred by commensal microbiota on their hosts. Clinical microbiota analysis and animal studies in germ-free or antibiotic-treated mice have been crucial for improving our understanding of the role of the microbiome on the host mucosal surface; however, studies on the mechanisms involved in microbiome-host interactions remain limited to small animal models. Here, we demonstrated that rhesus monkeys under short-term broad-spectrum antibiotic treatment could be used as a model to study the gut mucosal host-microbiome niche and immune balance with steady health status. Results showed that the diversity and community structure of the gut commensal bacteria in rhesus monkeys were both disrupted after antibiotic treatment. Furthermore, the 16S rDNA amplicon sequencing results indicated that Escherichia-Shigella were predominant in stool samples 9 d of treatment, and the abundances of bacterial functional genes and predicted KEGG pathways were significantly changed. In addition to inducing aberrant morphology of small intestinal villi, the depletion of gut commensal bacteria led to increased proportions of CD3 + T, CD4 + T, and CD16 + NK cells in peripheral blood mononuclear cells (PBMCs), but decreased numbers of Treg and CD20 + B cells. The transcriptome of PBMCs from antibiotic-treated monkeys showed that the immune balance was affected by modulation of the expression of many functional genes, including IL-13, VCAM1, and LGR4. |
| Databáze: | OpenAIRE |
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