Screening Plasma Donors for High-Titre Antibody to Cytomegalovirus Using a Latex Agglutination Test
Autor: | A. Al-Izzi, Marcela Contreras, John A. J. Barbara, Craig M. McDonald |
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Rok vydání: | 1990 |
Předmět: |
Adult
Male medicine.medical_treatment Congenital cytomegalovirus infection Cytomegalovirus Blood Donors Antibodies Viral Plasma Direct agglutination test Blood plasma medicine Humans Mass Screening biology business.industry Plasmapheresis Hematology General Medicine Middle Aged medicine.disease Complement fixation test Latex fixation test Titer Immunology biology.protein Female Antibody business Latex Fixation Tests |
Zdroj: | Vox Sanguinis. 59:83-85 |
ISSN: | 1423-0410 0042-9007 |
Popis: | Cytomegalovirus (CMV) can cause severe morbidity in immunosuppressed patients. Regional transfusion centres in the UK are required to supply high-titre anti-CMV plasma to the Blood Products Laboratory (BPL), now called 'Bio-Products Laboratory', for the production of specific intravenous immunoglobulin at the Protein Fractionation Centre in Scotland. For this purpose, 703 plasmapheresis donors were screened by a modified latex agglutination test to assess their suitability as donors with high-titre anti-CMV. CMV antibodies were found in 48% of the donors. Seropositivity increased with age ranging from 33% in the 20- to 29-year age group to 57% in the 40-49 age group, although the 50-59 age group showed a slight decline to 53%. In all age ranges except the 40-49 group, seropositivity was highest in the female population. With the latex test, 14% of the total donors screened had a titre greater than or equal to 1 in 64, 7% greater than or equal to 1 in 128 and 4.2% greater than or equal to 1 in 256. Samples from 18 donors with titres greater than or equal to 1 in 128 were sent to BPL and all samples were found to have a sufficiently high titre for the production of CMV immune plasma. For BPL the 'cut-off' level for CMV immune plasma is a titre greater than or equal to 1 in 64 by complement fixation. Since the establishment of a panel of donors with high-titre anti-CMV plasma, more than 10 kg of plasma are dispatched monthly from our centre to BPL. |
Databáze: | OpenAIRE |
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