High Levels of Human Chorionic Gonadotropin (hCG) Correlate With Increased Aquaporin-9 (AQP9) Expression in Explants From Human Preeclamptic Placenta
| Autor: | Gabriela Inés Marino, M. Castro-Parodi, Alicia E. Damiano, Valeria Dietrich |
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| Rok vydání: | 2010 |
| Předmět: |
Adult
endocrine system medicine.medical_specialty Cell Survival Placenta Aquaporin Biology Aquaporins Chorionic Gonadotropin Human chorionic gonadotropin Preeclampsia Young Adult Syncytiotrophoblast Pre-Eclampsia Downregulation and upregulation Pregnancy Internal medicine Cyclic AMP medicine Humans Cells Cultured reproductive and urinary physiology Gene Expression Regulation Developmental Obstetrics and Gynecology Apical membrane medicine.disease Adenosine Up-Regulation Endocrinology medicine.anatomical_structure embryonic structures Female hormones hormone substitutes and hormone antagonists Signal Transduction medicine.drug |
| Zdroj: | Reproductive Sciences. 17:444-453 |
| ISSN: | 1933-7205 1933-7191 |
| DOI: | 10.1177/1933719110361385 |
| Popis: | Trophoblastic abnormalities have a central role in the pathophysiology of preeclampsia, and some placental hormones, such as human chorionic gonadotropin (hCG), could affect the placental function. Here, we hypothesized that the elevated serum levels of hCG may be involved in the increased aquaporin-9 (AQP9) protein expression in preeclamptic placentas via adenosine 3('),5(')-cyclic phosphate (cAMP) pathways. Normal placental explants were cultured with different concentrations of recombinant hCG or 8-Br-cAMP, a potent analogue of cAMP. We evaluated AQP9 protein expression and localization. After both treatments, we localized AQP9 in the apical membrane of syncytiotrophoblast and in the cytoplasm. We also observed a concentration-dependent effect on AQP9 protein expression. In addition, water uptake increased 1.6-fold in explants treated with hCG. Our results suggest that hCG may increase AQP9 protein expression and functionality via cAMP pathways. Although, in preeclamptic placentas high levels of hCG may upregulate AQP9 protein expression, AQP9 functionality was reduced possibly by other factors. |
| Databáze: | OpenAIRE |
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