Quercetin attenuates mitochondrial dysfunction and biogenesis via upregulated AMPK/SIRT1 signaling pathway in OA rats
Autor: | Li-Nan Qiu, Xiao-Juan Chen, Yu-Ju Luo |
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Rok vydání: | 2018 |
Předmět: |
Male
0301 basic medicine Pharmacology Mitochondrion DNA Mitochondrial Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound Chondrocytes 0302 clinical medicine Sirtuin 1 Osteoarthritis medicine Animals heterocyclic compounds Membrane Potential Mitochondrial chemistry.chemical_classification Reactive oxygen species Organelle Biogenesis biology Chemistry Glutathione peroxidase Adenylate Kinase AMPK General Medicine Glutathione Adenosine Mitochondria Up-Regulation 030104 developmental biology biology.protein Quercetin Signal transduction Reactive Oxygen Species 030217 neurology & neurosurgery Signal Transduction medicine.drug |
Zdroj: | Biomedicine & Pharmacotherapy. 103:1585-1591 |
ISSN: | 0753-3322 |
DOI: | 10.1016/j.biopha.2018.05.003 |
Popis: | Despite the severity of osteoarthritis (OA), current medical therapy strategies for OA aim at symptom control and pain reduction, as there is no ideal drug for effective OA treatment. OA rat model was used to explore the therapeutic function of quercetin on remission of OA, by determining the reactive oxygen species (ROS) levels, mitochondrial function and extracellular matrix integrity. Quercetin could attenuate ROS generation and augment the glutathione (GSH) and glutathione peroxidase (GPx) expression levels in OA rat. Quercetin not only enhanced mitochondrial membrane potential, oxygen consumption, adenosine triphosphate (ATP) levels in mitochondria, but also increased the mitochondrial copy number. Furthermore, the interlukin (IL)-1β-induced accumulation of nitric oxide (NO), matrixmetalloproteinase (MMP)-3) and MMP-13 could be suppressed by quercetin. Finally, we confirmed that the therapeutic properties of quercetin on OA might function through the adenosine monophosphate-activated protein kinase/sirtuin 1 (AMPK/SIRT1) signaling pathway. In summary, quercetin could alleviate OA through attenuating the ROS levels, reversing the mitochondrial dysfunction and keeping the integrality of extracellular matrix of joint cartilage. The underlying mechanism might involve the regulation of AMPK/SIRT1 signaling pathway. |
Databáze: | OpenAIRE |
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