The effect of cyclo-DfKRG peptide immobilization on titanium on the adhesion and differentiation of human osteoprogenitor cells
| Autor: | Stéphane Pallu, Joelle Amédée-Vilamitjana, Andreas Sewing, Michel Dard, Christine Labrugère, Chantal Bourget, Michel Vernizeau, Marie-Christine Durrieu, Alfred Jonczyk, Reine Bareille |
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| Rok vydání: | 2004 |
| Předmět: |
Materials science
Biophysics chemistry.chemical_element Bioengineering Peptide Peptides Cyclic Biomaterials chemistry.chemical_compound Coated Materials Biocompatible Osteogenesis Materials Testing medicine Cell Adhesion Humans Cell adhesion Cells Cultured chemistry.chemical_classification Titanium Osteoblasts Osteoblast Cell Differentiation Adhesion Grafting Hematopoietic Stem Cells Phosphonate medicine.anatomical_structure chemistry Biochemistry Mechanics of Materials Ceramics and Composites Thiol Adsorption Protein Binding |
| Zdroj: | Biomaterials. 26(34) |
| ISSN: | 0142-9612 |
| Popis: | This study takes place in the field of development of a bioactive surface of titanium alloys. In this paper, titanium was functionalized with cyclo-DfKRG peptide by coating or grafting using different anchors (thiol or phosphonate) as spacers between the surface and the peptide. Cell adhesion, and differentiation of human osteoprogenitor (HOP) cells arising from human bone marrow were investigated. Our results seem to demonstrate that cyclo-DfKRG peptide coating with a phosphonate anchor and grafting procedure contributes to higher cell adhesion and a strong ALP and Cbfa1 mRNA expression, after 10 days of cell seeding. At the contrary, this peptide coated with a thiol anchor stimulates differentiation of HOP within 3 days of culture. |
| Databáze: | OpenAIRE |
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