Exploring human plasma proteome strategies: high efficiency in-solution digestion protocol for multi-dimensional protein identification technology

Autor:	Luwang Andy Zhu, Richard A. Katenhusen, Qinhua Cindy Ru, Jordan Silberman, Henry Brzeski, Craig D. Shriver, Michael N. Liebman
Rok vydání:	2005
Předmět:	Protocol (science) Chromatography Proteome Chemistry Gene Expression Profiling Organic Chemistry General Medicine Blood Proteins Biochemistry Analytical Chemistry Solutions Digestion (alchemy) Human plasma Multi dimensional Humans Protein identification
Zdroj:	Journal of chromatography. A. 1111(2)
ISSN:	0021-9673
Popis:	Multi-dimensional protein identification technology (MudPIT) is becoming a prevalent proteomic approach due to its high-throughput separations and accurate mass detection. Prior to MudPIT analysis, complicated samples required in-solution digestion. Unlike in-gel digestion, in which enzymes work on just a few proteins, in-solution digestion involves simultaneous digestion of hundreds or thousands of proteins. In-solution digestion protocols must therefore be very efficient. Few investigations have evaluated the efficiency of in-solution digestion protocols. The present research compared three such protocols. Results suggest that a protocol utilizing trifluoroethanol (TFE) as denaturant is most efficient.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1c0fc9be623c1671eacf8338946e339e https://pubmed.ncbi.nlm.nih.gov/16569577 Zobrazit plný text záznamu Full Text from ScienceDirect