Mobilization and collection of CD34+ cells for autologous transplantation of peripheral blood hematopoietic progenitor cells in children: analysis of two different granulocyte-colony stimulating factor doses
| Autor: | Simone dos Santos Aguiar, Katia A.B. Eid, Eliana C M Miranda |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Autologous transplantation
medicine.medical_specialty Mobilization lcsh:RC633-647.5 business.industry Hematopoietic stem cell mobilization CD34 Urology Granulocyte colony-stimulatingfactor lcsh:Diseases of the blood and blood-forming organs Hematology Leukapheresis Granulocyte colony-stimulating factor Surgery Apheresis Hematopoietic progenitor cells Medicine Original Article business Hematopoietic Stem Cell Mobilization |
| Zdroj: | Revista Brasileira de Hematologia e Hemoterapia Revista Brasileira de Hematologia e Hemoterapia, Vol 37, Iss 3, Pp 160-166 (2015) Revista Brasileira de Hematologia e Hemoterapia v.37 n.3 2015 Revista brasileira de hematologia e hemoterapia Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular (ABHHTC) instacron:ABHHTC Revista Brasileira de Hematologia e Hemoterapia, Volume: 37, Issue: 3, Pages: 160-166, Published: JUN 2015 |
| ISSN: | 1516-8484 |
| DOI: | 10.1016/j.bjhh.2015.02.006 |
| Popis: | Introduction: The use of peripheral hematopoietic progenitor cells (HPCs) is the cell choice in autologous transplantation. The classic dose of granulocyte-colony stimulating factor (G- CSF) for mobilization is a single daily dose of 10 µg/kg of patient body weight. There is a theory that higher doses of granulocyte-colony stimulating factor applied twice daily could increase the number of CD34+ cells collected in fewer leukapheresis procedures. Objective: The aim of this study was to compare a fractionated dose of 15 µg G-CSF/kg of body weight and the conventional dose of granulocyte-colony stimulating factor in respect to the number of leukapheresis procedures required to achieve a minimum collection of 3 × 106 CD34+ cells/kg body weight. Methods: Patients were divided into two groups: Group 10 - patients who received a single daily dose of 10 µg G-CSF/kg body weight and Group 15 - patients who received a fractioned dose of 15 µg G-CSF/kg body weight daily. The leukapheresis procedure was carried out in an automated cell separator. The autologous transplantation was carried out when a minimum number of 3 × 106 CD34+ cells/kg body weight was achieved. Results: Group 10 comprised 39 patients and Group 15 comprised 26 patients. A total of 146 apheresis procedures were performed: 110 (75.3%) for Group 10 and 36 (24.7%) for Group 15. For Group 10, a median of three (range: 1-7) leukapheresis procedures and a mean of 8.89 × 106 CD34+ cells/kg body weight (±9.59) were collected whereas for Group 15 the corresponding values were one (range: 1-3) and 5.29 × 106 cells/kg body weight (±4.95). A statistically significant difference was found in relation to the number of apheresis procedures (p-value |
| Databáze: | OpenAIRE |
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