Overexpression of a Profilin (GhPFN2) Promotes the Progression of Developmental Phases in Cotton Fibers
| Autor: | Li-Bo Han, Gai-Li Jiao, Hai-Yun Wang, Juan Wang, Pi-Ming Zhao, Shanjin Huang, Gui-Xian Xia, Yiyan Zheng |
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| Rok vydání: | 2010 |
| Předmět: |
Physiology
Molecular Sequence Data Plant Science Biology Microtubules Profilins Cell Wall Gene Expression Regulation Plant Microtubule Amino Acid Sequence Cotton Fiber Cloning Molecular Cytoskeleton Actin Plant Proteins Gossypium Cell growth Gene Expression Regulation Developmental Cell Biology General Medicine Plants Genetically Modified Plant cell Actin cytoskeleton Actins Cell biology Biochemistry Profilin RNA Plant biology.protein Elongation |
| Zdroj: | Plant and Cell Physiology. 51:1276-1290 |
| ISSN: | 1471-9053 0032-0781 |
| DOI: | 10.1093/pcp/pcq086 |
| Popis: | Cotton fi ber development at the stages of elongation and secondary wall synthesis determines the traits of fi ber length and strength. To date, the mechanisms controlling the progression of these two phases remain elusive. In this work, the function of a fi ber-preferential actin-binding protein (GhPFN2) was characterized by cytological and molecular studies on the fi bers of transgenic green-colored cotton ( Gossypium hirsutum ) through three successive generations. Overexpression of GhPFN2 caused pre-terminated cell elongation, resulting in a marked decrease in the length of mature fi bers. Cytoskeleton staining and quantitative assay revealed that thicker and more abundant F-actin bundles formed during the elongation stage in GhPFN2 -overexpressing fi bers. Accompanying this alteration, the developmental reorientation of transverse microtubules to the oblique direction was advanced by 2 d at the period of transition from elongation to secondary wall deposition. Birefringence and reverse transcription–PCR analyses showed that earlier onset of secondary wall synthesis occurred in parallel. These data demonstrate that formation of the higher actin structure plays a determinant role in the progression of developmental phases in cotton fi bers, and that GhPFN2 acts as a critical modulator in this process. Such a function of the actin cytoskeleton in cell phase conversion may be common to other secondary wall-containing plant cells. |
| Databáze: | OpenAIRE |
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