Two simultaneous hepatitis B virus epidemics among injecting drug users and men who have sex with men in Buenos Aires, Argentina: characterization of the first D/A recombinant from the American continent
| Autor: | L. A. Peralta, Jose Raul Oubiña, María Cecilia Bobillo, Veronica Lidia Mathet, María Laura Minassian, D. O. Sanchez, Mercedes Weissenbacher, Cintia Wanda Rivero, Peter Simmonds, Jorge Benetucci, María M. Avila, Pablo Daniel Ghiringhelli, Yasuhito Tanaka, Marcela Segura, Masashi Mizokami, María Luján Cuestas, Daniel Corach, E. D. Gimenez, Fuat Kurbanov, Julieta Trinks |
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| Rok vydání: | 2008 |
| Předmět: |
Serotype
Adult Male HBsAg Hepatitis B virus Genotype Population Argentina medicine.disease_cause Polymerase Chain Reaction Serology Men who have sex with men Drug Users Virology medicine Prevalence Cluster Analysis Humans Homosexuality Male education Substance Abuse Intravenous Phylogeny Recombination Genetic education.field_of_study Molecular Epidemiology Hepatology business.industry virus diseases Sequence Analysis DNA Hepatitis B Middle Aged medicine.disease digestive system diseases Infectious Diseases Immunology DNA Viral Female business Polymorphism Restriction Fragment Length |
| Zdroj: | Journal of viral hepatitis. 15(11) |
| ISSN: | 1365-2893 |
| Popis: | Previous studies have revealed that hepatitis B virus (HBV)/D and HBV/F predominate among blood donors from Buenos Aires, Argentina. In the present study, blood samples from two high-risk groups were analysed: 160 corresponding to street- and hospital-recruited injecting drug users [81.2% showing the 'anti-hepatitis B core antigen (anti-HBc) only' serological pattern] and 20 to hepatitis B surface antigen (HBsAg)(+)/anti-HBc(+) men who have sex with men. HBV genotypes were assigned by polymerase chain reaction amplification followed by restriction fragment length polymorphism and confirmed by nucleotide sequencing of two different coding regions. HBV DNA was detected in 27 injecting drug users (16.9%, occult infection prevalence: 7.7%), and 14 men who have sex with men (70%). HBV/A prevailed among injecting drug users (81.8%) while HBV/F was predominant among men who have sex with men (57.1%). The high predominance of HBV/A among injecting drug users is in sharp contrast to its low prevalence among blood donors (P = 0.0006) and men who have sex with men (P = 0.0137). Interestingly, all HBV/A S gene sequences obtained from street-recruited injecting drug users encoded the rare serotype ayw1 and failed to cluster within any of the known A subgenotypes. Moreover, one of the HBV strains from a hospital-recruited injecting drug user was fully sequenced and found to be the first completely characterized D/A recombinant genome from the American continent. Data suggest that two simultaneous and independent HBV epidemics took place in Buenos Aires: one spreading among injecting drug users and another one sexually transmitted among the homosexual and heterosexual population. |
| Databáze: | OpenAIRE |
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