Genotoxic Response and Mortality in 3 Marine Copepods Exposed to Waterborne Copper
| Autor: | Josefin Titelman, Torben Lode, Katrine Borgå, Jan Heuschele, Ketil Hylland, Andrea Silke Sahlmann |
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| Rok vydání: | 2019 |
| Předmět: |
Aquatic Organisms
Health Toxicology and Mutagenesis ved/biology.organism_classification_rank.species 010501 environmental sciences medicine.disease_cause 01 natural sciences Copepoda 03 medical and health sciences medicine Animals Environmental Chemistry Trace metal 030304 developmental biology 0105 earth and related environmental sciences Acartia tonsa 0303 health sciences biology Chemistry ved/biology Body Weight Pelagic zone Hydrogen Peroxide Marine invertebrates biology.organism_classification Acute toxicity Comet assay Environmental chemistry Copper Water Pollutants Chemical Genotoxicity Copepod DNA Damage Mutagens |
| Zdroj: | Environmental Toxicology and Chemistry. 38:2224-2232 |
| ISSN: | 1552-8618 0730-7268 |
| DOI: | 10.1002/etc.4541 |
| Popis: | Copper (Cu) is an essential trace metal, but may also be toxic to aquatic organisms. Although many studies have investigated the cytotoxicity of Cu, little is known about the in vivo genotoxic potential of Cu in marine invertebrates. We investigated the genotoxicity of Cu in 2 pelagic calanoid copepods, Acartia tonsa and Temora longicornis, and the intertidal harpacticoid copepod Tigriopus brevicornis by exposing them for 6 and 72 h to waterborne Cu (0, 6, and 60 µg Cu/L). A subsequent 24-h period in filtered seawater was used to investigate delayed effects or recovery. Genotoxicity was evaluated as DNA strand breaks in individual copepods using the comet assay. Copper did not increase DNA strand breaks in any of the species at any concentration or time point. The treatment did, however, cause 100% mortality in A. tonsa following exposure to 60 µg Cu/L. Acartia tonsa and T. longicornis were more susceptible to Cu-induced mortality than the benthic harpacticoid T. brevicornis, which appeared to be unaffected by the treatments. The results show major differences in Cu susceptibility among the 3 copepods and also that acute toxicity of Cu to A. tonsa is not directly associated with genotoxicity. We also show that the comet assay can be used to quantify genotoxicity in individual copepods. Environ Toxicol Chem 2019;38:2224-2232. © 2019 SETAC. |
| Databáze: | OpenAIRE |
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