Engineering prolonged-acting prodrugs employing an albumin-binding probe that undergoes slow hydrolysis at physiological conditions
| Autor: | Yonit Marcus, Vered Lev-Goldman, Keren Sasson, Yoram Shechter, S. Rubinraut, Mati Fridkin |
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| Rok vydání: | 2010 |
| Předmět: |
Stereochemistry
Molecular Sequence Data Serum albumin Pharmaceutical Science Peptide Mice chemistry.chemical_compound Hydrolysis In vivo Animals Humans Hypoglycemic Agents Insulin Prodrugs Amino Acid Sequence Derivatization Serum Albumin chemistry.chemical_classification biology Venoms Chemistry Aminoglycoside Albumin Prodrug Rats Glucose biology.protein Exenatide Peptides Protein Binding |
| Zdroj: | Journal of Controlled Release. 142:214-220 |
| ISSN: | 0168-3659 |
| DOI: | 10.1016/j.jconrel.2009.10.028 |
| Popis: | Here we describe the design and application of OSu-FMS-MAL-S-(CH(2))(15)-COOH, an agent that associates with albumin while linked to a peptide or a protein with sufficient affinity (Ka=2 to 2.6 x 10(5)M(-1)) to protract the action of short- lived peptides and proteins in vivo. Under physiological conditions this probe undergoes spontaneous hydrolysis with the concomitant reactivation of inactive conjugates. Intravenously administered (125)I-labeled-Insulin-FMS-MAL-S-(CH(2))(15)-COOH to rats shows half-life of 17+/-2h, exceeding 5.2 times that obtained with intravenously administered (125)I-labeled Insulin. In mice this derivative facilitates glucose-lowering effect over a period of 24h, yielding AUC five times greater than that obtained by a similar dose of insulin-detemir. Similarly, subcutaneous administration of Exendin-4-FMS-MAL-S-(CH(2))(15)-COOH into mice facilitated prolonged and stable reduction in glucose level, yielding a t(1/2) value of 28+/-2h, exceeding the effect of exendin-4 4.7 folds. The inactive derivative gentamicin-FMS-MAL-S-(CH(2))(15)-COOH regained its full antibacterial potency upon incubation at physiological conditions yielding a t(1/2) value of 7.1+/-0.2h. In conclusion, the albumin-binding probe we introduced enables to prolong the action of any amino containing molecule in vivo, without the drawback of inactivation that often occurs upon such derivatization. |
| Databáze: | OpenAIRE |
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