Rhodomyrtone decreases Staphylococcus aureus SigB activity during exponentially growing phase and inhibits haemolytic activity within membrane vesicles
| Autor: | Watcharapong Mitsuwan, Irene Jiménez-Munguía, Monton Visutthi, Wipawadee Sianglum, Manuel J. Rodríguez-Ortega, Supayang P. Voravuthikunchai, A. Jover, F. Barcenilla, M. García, M. Pujol, O. Gasch, M.A. Domínguez, M. Camoez, C. Dueñas, E. Ojeda, J.A. Martínez, F. Marco, F. Chaves, M. Lagarde, F. López-Medrano, J.M. Montejo, E. Bereciertua, J.L. Hernández, M.Á. von Wichmann, A. Goenaga, J.M. García-Arenzana, B. Padilla, C. Padilla, E. Cercenado, G. García-Prado, J. Tapiol, J.P. Horcajada, M. Montero, M. Salvadó, A. Arnáiz, C. Fernández, E. Calbo, M. Xercavins, A. Granados, D. Fontanals, V. Pintado, E. Loza, J. Torre-Cisneros, R. Lara, F. Rodríguez-López, C. Natera, J.R. Blanco, I. Olarte, N. Benito, B. Mirelis, J. Murillas, E. Ruiz de Gopegui, H. Espejo, M.A. Morera, J. Rodríguez-Baño, L.E. López-Cortés, A. Pascual, C. Martín, J.A. Lepe, J. Molina, R. Sordé, B. Almirante, N. Larrosa |
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| Přispěvatelé: | Thailand Research Fund, Universidad de Córdoba (España) |
| Rok vydání: | 2019 |
| Předmět: |
Methicillin-Resistant Staphylococcus aureus
0301 basic medicine Staphylococcus aureus SigB Virulence Factors Xanthones 030106 microbiology Virulence Sigma Factor Microbial Sensitivity Tests Rhodomyrtone medicine.disease_cause Microbiology 03 medical and health sciences Minimum inhibitory concentration Bacterial Proteins Vancomycin Sigma factor medicine Humans Secretion Minimum bactericidal concentration biology Chemistry Cell Membrane Staphylococcal Infections biochemical phenomena metabolism and nutrition biology.organism_classification Membrane vesicles Anti-Bacterial Agents DNA-Binding Proteins Haemolytic activity 030104 developmental biology Infectious Diseases Mutation bacteria Bacteria medicine.drug |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname MICROBIAL PATHOGENESIS r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau |
| ISSN: | 0882-4010 |
| DOI: | 10.1016/j.micpath.2018.12.019 |
| Popis: | REIPI/GEIH Study Group. Sigma factor B (SigB) controls the expression of Staphylococcus aureus genes including virulence factors and plays a role in the bacterial secretion system through membrane vesicle production. Inhibition of SigB could attenuate SigB dependent virulence and secretion system. The objective of this study was to determine the effects of rhodomyrtone on SigB and virulence factors related to SigB. Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) values of rhodomyrtone against 67 clinical methicillin-resistant S. aureus isolates were 0.25–8 μg/ml, which were similar to those of vancomycin. Using luciferase gene fused to SigB dependent promoters of asp23, five time reduction in SigB activity was observed when the bacteria were treated with rhodomyrtone for 3 h. Rhodomyrtone significantly reduced SigB activity in a concentration dependent manner in exponentially growing cells (P This work was financially supported by The Royal Golden Jubilee Ph.D. Program (Grant No. PHD/0033/2553) and TRF Senior Research Scholar (Grant No. RTA 6180006), the Thailand Research Fund. M.J.R.O. was funded by the 'XXII Programa Propio de Fomento de la Investigación', University of Córdoba. |
| Databáze: | OpenAIRE |
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