Recombinant human macrophage colony-stimulating factor augments pulmonary host defences against Aspergillus fumigatus
| Autor: | Corina E. Gonzalez, Sondra Lee, Maria Tsokos, Erwin Feuerstein, John Bacher, Thomas J. Walsh, Caron A. Lyman, Carla Del Guercio, Andreas Gehrt, Emmanuel Roilides |
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| Rok vydání: | 2001 |
| Předmět: |
Macrophage colony-stimulating factor
Antifungal Agents medicine.medical_treatment Phagocytosis Immunology Neutropenia Biochemistry Aspergillus fumigatus In vivo medicine Immunology and Allergy Animals Aspergillosis Humans Molecular Biology biology Lung Diseases Fungal Pulmonary Infarction Macrophage Colony-Stimulating Factor Macrophages Hematology Pneumonia biology.organism_classification medicine.disease Recombinant Proteins Pulmonary Alveoli Survival Rate Cytokine Female Rabbits Pulmonary Embolism Ex vivo |
| Zdroj: | Cytokine. 15(2) |
| ISSN: | 1043-4666 |
| Popis: | The in vivo and ex vivo effects of macrophage colony-stimulating factor (M-CSF) were studied in a profoundly neutropenic rabbit model in order to determine its potential to augment pulmonary host defence against Aspergillus. M-CSF (100-600 microg/kg/d) was administered prophylactically to neutropenic rabbits with pulmonary aspergillosis starting three days pre-inoculation and then throughout neutropenia. Rabbits receiving M-CSF had significantly increased survival (P=0.01) and decreased pulmonary injury, as measured by decreased pulmonary infarction (P=0.004), when compared with untreated controls. Microscopic studies demonstrated greater numbers of activated pulmonary alveolar macrophages (PAMs) in lung tissue of rabbits receiving M-CSF, in comparison to controls (P0.001). PAMs harvested from rabbits treated with M-CSF had a significantly greater percent phagocytosis of Aspergillus fumigatus conidia than did PAMs from controls (P=0.04). These data indicate that prophylactic administration of M-CSF augments pulmonary host defence against A. fumigatus and suggest a potential role for this cytokine as adjunctive therapy in the treatment of pulmonary aspergillosis in the setting of profound neutropenia. |
| Databáze: | OpenAIRE |
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