Diagnosis of paroxysmal nocturnal haemoglobinuria by phenotypic analysis of erythrocytes using two-colour flow cytometry with monoclonal antibodies to DAF and CD59/MACIF
| Autor: | Chokichi Hashimoto, Tsutomu Shichishima, Hitoshi Ohto, Yukio Maruyama, Takashi Terasawa, Yurie Saitoh |
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| Rok vydání: | 1993 |
| Předmět: |
Adult
Male Erythrocytes medicine.drug_class Population Hemoglobinuria Paroxysmal CD59 Antigens CD59 Biology Monoclonal antibody Flow cytometry Immunophenotyping Diagnosis Differential Antigens CD hemic and lymphatic diseases medicine Humans education Decay-accelerating factor Aged education.field_of_study Complement Inactivator Proteins Membrane Glycoproteins medicine.diagnostic_test CD55 Antigens Mean fluorescence intensity Antibodies Monoclonal Hematology Middle Aged Flow Cytometry Molecular biology Hematologic Diseases Red blood cell medicine.anatomical_structure Immunology Female Paroxysmal nocturnal haemoglobinuria |
| Zdroj: | British journal of haematology. 85(2) |
| ISSN: | 0007-1048 |
| Popis: | Summary We investigated the relationship between the complement lysis sensitivity test and two-colour flow cyto-metric analysis using monoclonal antibodies to decay accelerating factor (DAF) and CD59/membrane attack complex inhibitory factor (MACIF) in patients with paroxysmal nocturnal haemoglobinuria (PNH) and other haematological diseases. Flow cytometry showed that all 59 PNH patients had two or three erythrocyte populations, while all 74 patients with other haematological diseases and all 31 healthy volunteers had a single erythrocyte population. We compared the percentage of PNH III erythrocytes in the lysis test with the percentage of negative cells shown by flow cytometry in 52 PNH patients, and found a significant correlation (r=0–960, P |
| Databáze: | OpenAIRE |
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