Rhomboid intramembrane protease YqgP licenses bacterial membrane protein quality control as adaptor of FtsH AAA protease

Autor: Rozálie Hexnerová, Mathieu Baudet, Thierry Doan, Jordan Delisle, Anne Galinier, Baptiste Cordier, Jakub Began, Vaclav Veverka, Kvido Strisovsky, Milan Kožíšek, Petra Rampírová, Yohann Couté, Jana Březinová, Pavel Srb
Přispěvatelé: Laboratoire de chimie bactérienne (LCB), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences (IOCB / CAS), Czech Academy of Sciences [Prague] (CAS), Laboratoire de Biochimie des Systèmes Perturbés, Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Etude de la dynamique des protéomes (EDyP ), Laboratoire de Biologie à Grande Échelle (BGE - UMR S1038), Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA)
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Proteases
Intramembrane protease
Magnesium transporter
medicine.medical_treatment
Bacillus subtilis
Endoplasmic-reticulum-associated protein degradation
General Biochemistry
Genetics and Molecular Biology
Article
ER-associated degradation
03 medical and health sciences
0302 clinical medicine
rhomboid
medicine
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
Molecular Biology
030304 developmental biology
membrane transporter
0303 health sciences
Protease
proteostasis
General Immunology and Microbiology
biology
General Neuroscience
Rhomboid protease
Rhomboid
Post-translational Modifications
Proteolysis & Proteomics
Articles
Protein Biosynthesis & Quality Control
biology.organism_classification
Microbiology
Virology & Host Pathogen Interaction
Cell biology
ER‐associated degradation
biology.protein
030217 neurology & neurosurgery
intramembrane protease
Zdroj: EMBO Journal
EMBO Journal, 2020, ⟨10.15252/embj.2019102935⟩
EMBO Journal, EMBO Press, 2020, ⟨10.15252/embj.2019102935⟩
The EMBO Journal
ISSN: 0261-4189
1460-2075
DOI: 10.15252/embj.2019102935⟩
Popis: Magnesium homeostasis is essential for life and depends on magnesium transporters, whose activity and ion selectivity need to be tightly controlled. Rhomboid intramembrane proteases pervade the prokaryotic kingdom, but their functions are largely elusive. Using proteomics, we find that Bacillus subtilis rhomboid protease YqgP interacts with the membrane‐bound ATP‐dependent processive metalloprotease FtsH and cleaves MgtE, the major high‐affinity magnesium transporter in B. subtilis. MgtE cleavage by YqgP is potentiated in conditions of low magnesium and high manganese or zinc, thereby protecting B. subtilis from Mn2+/Zn2+ toxicity. The N‐terminal cytosolic domain of YqgP binds Mn2+ and Zn2+ ions and facilitates MgtE cleavage. Independently of its intrinsic protease activity, YqgP acts as a substrate adaptor for FtsH, a function that is necessary for degradation of MgtE. YqgP thus unites protease and pseudoprotease function, hinting at the evolutionary origin of rhomboid pseudoproteases such as Derlins that are intimately involved in eukaryotic ER‐associated degradation (ERAD). Conceptually, the YqgP‐FtsH system we describe here is analogous to a primordial form of “ERAD” in bacteria and exemplifies an ancestral function of rhomboid‐superfamily proteins.
The unprecedented pseudoprotease function of a prokaryotic rhomboid family in degradation of a magnesium transporter member reveals striking analogies to eukaryotic ERAD quality control systems.
Databáze: OpenAIRE
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