Thioredoxin m4 Controls Photosynthetic Alternative Electron Pathways in Arabidopsis
| Autor: | Dominique Rumeau, Anne-Claire Cazalé, Pascal Rey, Agathe Courteille, Immaculada Farran, Michel Havaux, Ruth Sanz-Barrio, Simona Vesa, Noelle Becuwe-Linka |
|---|---|
| Přispěvatelé: | Unite Mixte Rech Biol Vegetale & Microbiol Enviro, Centre National de la Recherche Scientifique (CNRS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Université de la Méditerranée - Aix-Marseille 2, Universidad de Navarra [Pamplona] (UNAV), Unité mixte de recherche interactions plantes-microorganismes, Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
0106 biological sciences
Photoinhibition animal structures Physiology [SDV]Life Sciences [q-bio] Plastoquinone TOBACCO CHLOROPLASTS Plant Science Biology Photosynthesis Photosystem I 01 natural sciences HIGHER-PLANTS 03 medical and health sciences chemistry.chemical_compound CHLOROPHYLL THERMOLUMINESCENCE DEHYDROGENASE-LIKE COMPLEX Arabidopsis AFTERGLOW LUMINESCENCE Genetics Arabidopsis thaliana [SDV.BV]Life Sciences [q-bio]/Vegetal Biology 030304 developmental biology GENE-EXPRESSION 0303 health sciences PLASTTERMINAL OXIDASE TARGET PROTEINS food and beverages biology.organism_classification Cell biology Chloroplast Biochemistry chemistry PHOTOSYSTEM-I C-3 PLANTS Thioredoxin 010606 plant biology & botany |
| Zdroj: | Plant Physiology Plant Physiology, American Society of Plant Biologists, 2013, 161 (1), pp.508-520. ⟨10.1104/pp.112.207019⟩ Plant Physiology, 2013, 161 (1), pp.508-520. ⟨10.1104/pp.112.207019⟩ Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 0032-0889 1532-2548 |
| DOI: | 10.1104/pp.112.207019⟩ |
| Popis: | In addition to the linear electron flow, a cyclic electron flow (CEF) around photosystem I occurs in chloroplasts. In CEF, electrons flow back from the donor site of photosystem I to the plastoquinone pool via two main routes: one that involves the Proton Gradient Regulation5 (PGR5)/PGRL1 complex (PGR) and one that is dependent of the NADH dehydrogenase-like complex. While the importance of CEF in photosynthesis and photoprotection has been clearly established, little is known about its regulation. We worked on the assumption of a redox regulation and surveyed the putative role of chloroplastic thioredoxins (TRX). Using Arabidopsis (Arabidopsis thaliana) mutants lacking different TRX isoforms, we demonstrated in vivo that TRXm4 specifically plays a role in the down-regulation of the NADH dehydrogenase-like complex-dependent plastoquinone reduction pathway. This result was confirmed in tobacco (Nicotiana tabacum) plants overexpressing the TRXm4 orthologous gene. In vitro assays performed with isolated chloroplasts and purified TRXm4 indicated that TRXm4 negatively controls the PGR pathway as well. The physiological significance of this regulation was investigated under steady-state photosynthesis and in the pgr5 mutant background. Lack of TRXm4 reversed the growth phenotype of the pgr5 mutant, but it did not compensate for the impaired photosynthesis and photoinhibition sensitivity. This suggests that the physiological role of TRXm4 occurs in vivo via a mechanism distinct from direct up-regulation of CEF. © 2012 American Society of Plant Biologists. All Rights Reserved. |
| Databáze: | OpenAIRE |
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