N-acetylcysteine dual and antagonistic effect on cadmium cytotoxicity in human leukemia cells
| Autor: | Nikola Skoupa, Petr Dolezel, K Kikalova, Petr Mlejnek, V. Maier |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Cell Survival
Health Toxicology and Mutagenesis chemistry.chemical_element 010501 environmental sciences Pharmacology Toxicology medicine.disease_cause 01 natural sciences Acetylcysteine 03 medical and health sciences medicine Cytotoxic T cell Humans Cytotoxicity 030304 developmental biology 0105 earth and related environmental sciences Chelating Agents chemistry.chemical_classification 0303 health sciences Cadmium Reactive oxygen species U937 cell Dose-Response Relationship Drug General Medicine U937 Cells chemistry Environmental Pollutants K562 Cells Reactive Oxygen Species Oxidative stress medicine.drug K562 cells |
| Zdroj: | Environmental toxicology and pharmacology. 71 |
| ISSN: | 1872-7077 |
| Popis: | Although cadmium (Cd2+) is unable to form reactive oxygen species (ROS) directly, many of its adverse effects are connected to increased ROS generation resulting in cell death. In support of this supposition, a large number of studies have shown protective effects of antioxidants such as N-acetylcysteine (NAC) against cadmium induced cytotoxicity. Here, we describe the cytotoxic effects of Cd2+ on human leukemia U937 and K562 cells that were not mediated by oxidative stress. Surprisingly, we observed that addition of low concentrations of NAC can drastically potentiate cadmium cytotoxicity solely via ROS production. However, all adverse effects of the metal were prevented by NAC at high concentrations. Detailed analysis indicated that the protective effect of NAC was mediated by its ability to form stable complex with cadmium [Cd(NAC)2]. In conclusion, NAC exhibits dual and antagonistic effects on Cd2+ cytotoxicity in human leukemia cells. |
| Databáze: | OpenAIRE |
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