Cryptosporidium spp. CP15 and CSL protein-derived synthetic peptides’ immunogenicity and in vitro seroneutralisation capability
| Autor: | Jairo Oviedo, Fanny Guzmán, Joaquín Quílez, Gina Méndez-Callejas, Mark C. Jenkins, Catalina Avendaño, Manuel A. Patarroyo, Caridad Sánchez-Acedo |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Protozoan Vaccines
0301 basic medicine Swiss webster mouse Unclassified drug Mouse Enzyme linked immunosorbent assay Protozoan Proteins Antibodies Protozoan Cryptosporidiosis Antibody production In vivo study Synthesis 0302 clinical medicine Neutralizing antibody Priority journal Peptide vaccine Sporozoite surface antigen 15 vaccine Protozoal dna biology Immunogenicity Freund adjuvant Chemistry Infectious Diseases Cryptosporidium parvum Peptide Antibody response Vaccine immunogenicity Molecular Medicine Female Peptide analysis Antibody Protozoan vaccines Protozoan proteins Bioinformatics Sodium chloride Seroneutralization test Immunology 030231 tropical medicine Cp15 Cryptosporidium Antigens Protozoan Protozoal vaccine Protozoal protein Article Antibodies Microbiology Drug synthesis 03 medical and health sciences Immune system Antigen Drug formulation Csl Parasite antigen Animal experiment Antigens Protozoon antibody General Veterinary General Immunology and Microbiology protozoan Public Health Environmental and Occupational Health Péptidos Circumsporozoite like antigen vaccine In vitro study Dna DNA Protozoan Nonhuman biology.organism_classification Immunological procedures Antibody detection Humoral immunity 030104 developmental biology biology.protein Cell culture Peptides Controlled study Nucleotide sequence Vaccine Parásitos |
| Zdroj: | Repositorio EdocUR-U. Rosario Universidad del Rosario instacron:Universidad del Rosario Repositorio Institucional UDCA Universidad de Ciencias Aplicadas y Ambientales U.D.C.A instacron:Universidad de Ciencias Aplicadas y Ambientales U.D.C.A |
| ISSN: | 0264-410X |
| DOI: | 10.1016/j.vaccine.2018.09.044 |
| Popis: | Cryptosporidium spp. is a zoonotic intracellular protozoan and a significant cause of diarrhoea in humans and animals worldwide. This parasite can cause high morbidity in immunocompromised people and children in developing countries, livestock being the main reservoir. This study was aimed at performing preliminary tests on Swiss albino weaned mice (ICR) to evaluate the humoral immune response induced against peptides derived from Cryptosporidium parvum CP15 (15 kDa sporozoite surface antigen) and CSL (circumsporozoite-like antigen) proteins. Peptides were identified and characterised using bioinformatics tools and were chemically synthesised. The antibody response was determined and the neutralising effect of antibodies was measured in cell culture. Despite all peptides studied here were capable of stimulating antibody production, neutralising antibodies were detected for just two of the CP15-derived ones. Additional studies aimed at evaluating further the potential of such peptides as vaccine candidates are thus recommended. © 2018 Elsevier Ltd |
| Databáze: | OpenAIRE |
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