Cloning and Characterization of the Murine Genes for bHLH-ZIP Transcription Factors TFEC and TFEB Reveal a Common Gene Organization for All MiT Subfamily Members
| Autor: | Michael C. Ostrowski, A. Ian Cassady, David A. Hume, Nicole den Elzen, Michael Rehli |
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| Rok vydání: | 1999 |
| Předmět: |
Leucine zipper
Subfamily Molecular Sequence Data Restriction Mapping Basic helix-loop-helix leucine zipper transcription factors Biology Conserved sequence Evolution Molecular Mice Genetics Animals Humans Amino Acid Sequence Caenorhabditis elegans Conserved Sequence Genomic organization Leucine Zippers Base Sequence Basic helix-loop-helix Basic Helix-Loop-Helix Leucine Zipper Transcription Factors Helix-Loop-Helix Motifs Physical Chromosome Mapping Microphthalmia-associated transcription factor DNA-Binding Proteins TFEB Sequence Alignment Transcription Factors |
| Zdroj: | Genomics. 56:111-120 |
| ISSN: | 0888-7543 |
| DOI: | 10.1006/geno.1998.5588 |
| Popis: | The microphthalmia-TFE (MiT) subfamily of basic helix-loop-helix leucine zipper (bHLH-ZIP) transcription factors, including TFE3, TFEB, TFEC, and Mitf, has been implicated in the regulation of tissue-specific gene expression in several cell lineages. In this report, we investigate the genomic organization and structural relatedness of MiT transcription factors. We characterized the gene for mTFEC, which covers a region of more than 50 kb and is composed of seven exons. Further, we cloned a cDNA for the murine TFEB homologue and characterized its genomic structure. The eight coding exons of mTFEB are distributed over a 6-kb region. A multiple alignment of amino acid sequences of known MiT subfamily members indicates undescribed, conserved N-terminal regions and common putative phosphorylation sites for TFE3, TFEB, and Mitf. Also, intron-exon borders for characterized MiT genes appear completely conserved. A new family member and closely related putative transcription factor in Caenorhabditis elegans was identified by database searches that show a similar genomic organization within the bHLH-ZIP region and the acidic domain. Evolutionary aspects and implications for structure-function relationships are discussed. |
| Databáze: | OpenAIRE |
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