Construction of a highly bioluminescent Nitrosomonas as a probe for nitrification conditions
| Autor: | Hans-Georg Dr Rast, Karl O. Stetter, Susanne Ecker, Claudia Ludwig, Günther Eberz, Kerstin Schwindel |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Biology
Transfection Biochemistry Microbiology Ammonium Chloride chemistry.chemical_compound Genetics Bioluminescence Nitrosomonas Luciferases Molecular Biology Nitrites Nitrobacteraceae Nitrapyrin Genetic transfer Gene Transfer Techniques Thiourea General Medicine Hydrogen-Ion Concentration biology.organism_classification Culture Media Biodegradation Environmental chemistry Genes Bacterial Luminescent Measurements Picolines Nitrification Ammonium chloride Bacteria |
| Zdroj: | Archives of microbiology. 172(1) |
| ISSN: | 0302-8933 |
| Popis: | Cloned luciferase-encoding operons were transferred by conjugation to a natural isolate of the ammonia-oxidizing bacterial strain Nitrosomonas sp. RST41-3, thereby establishing conjugation as a tool for gene transfer into Nitrosomonas strains. Luminescence was dependent on the pH of the medium and the concentration of the substrate ammonium chloride. Moreover, the luminescence of the transconjugants was reduced immediately by micromolar concentrations of nitrapyrin and allylthiourea, which are specific inhibitors of nitrification. Our results indicate that luminescent Nitrosomonas strains may be useful as a probe to detect nitrification conditions in the natural environment as well as in sewage plants. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink