Heterozygosity of stromelysin-1 (rs3025058) promoter polymorphism is associated with gastric cancer
| Autor: | Nallari Pratibha, Amar Chand Bhayal, D. Krishnaveni, K P Shravan, Ananthapur Venkateshwari, Akka Jyothy |
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| Rok vydání: | 2015 |
| Předmět: |
Adult
Heterozygote Genotype India Single-nucleotide polymorphism Heterozygote advantage Middle Aged Biology Molecular biology Stromelysin 1 Loss of heterozygosity genomic DNA Oncology Risk Factors Stomach Neoplasms Agarose gel electrophoresis Humans Genetic Predisposition to Disease Matrix Metalloproteinase 3 Promoter Regions Genetic Genotyping Genetic Association Studies Aged |
| Zdroj: | Indian Journal of Cancer. 52:251 |
| ISSN: | 0019-509X |
| DOI: | 10.4103/0019-509x.175806 |
| Popis: | Background: Gastric cancer (GC) is the third most common cancer in India and is mediated by multiple genetic, epigenetic and environmental risk factors. A single nucleotide polymorphism rs3025058 at − 1171 of the stromelysin-1 (matrix metalloproteinase [MMP]-3) promoter is resulting due to insertion/deletion of adenine thought to have an impact on increasing the risk for tumor formation. Aim: This study is aimed to understand the role of stromelysin-1 rs3025058 (−1171, 5A/6A) promoter polymorphism in the etiology of GC in Indian population. Materials And Methods: Genomic DNA was isolated from blood samples of the GC patients and controls. The genotyping of stromelysin-1 rs3025058 (−1171, 5A/6A) promoter polymorphism was carried out by amplification refractory mutation system-polymerase chain reaction method followed by agarose gel electrophoresis. RESULTS: The frequency of 5A/5A, 5A/6A, and 6A/6A genotypes in GC patients were 7.69%, 76.92%, and 15.38%, while in controls were 5.31%, 86.73%, and7.96%, respectively. There was a significant difference in the distribution of 5A/6A genotype in patients compared to the controls (P < 0.05). Conclusion: This study showed an increased frequency of heterozygotes for stromelysin-1 rs3025058 and thought to be involved in the etiology of GC. |
| Databáze: | OpenAIRE |
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