Tuning the Loading and Release Properties of MicroRNA-Silencing Porous Silicon Nanoparticles by Using Chemically Diverse Peptide Nucleic Acid Payloads
| Autor: | Martina Neri, Jonathan M. Zuidema, Alessia Finotti, Jessica Gasparello, Alessandro Bertucci, Roberto Gambari, Roberto Corradini, Jinyoung Kang, Michael J. Sailor |
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| Rok vydání: | 2021 |
| Předmět: |
Peptide Nucleic Acids
Silicon release kinetics oligonucleotide mimics Oligonucleotides Biomedical Engineering Nanoparticle Peptide 02 engineering and technology Nanomaterials Biomaterials 03 medical and health sciences chemistry.chemical_compound anti-microRNA therapeutics nanomaterials 030304 developmental biology chemistry.chemical_classification 0303 health sciences Peptide nucleic acid Oligonucleotide drug delivery 021001 nanoscience & nanotechnology MicroRNAs chemistry Drug delivery Biophysics Nucleic acid Nanoparticles Chemical stability 0210 nano-technology Porosity |
| Zdroj: | ACS Biomaterials Science & Engineering. 8:4123-4131 |
| ISSN: | 2373-9878 |
| DOI: | 10.1021/acsbiomaterials.1c00431 |
| Popis: | Peptide nucleic acids (PNAs) are a class of artificial oligonucleotide mimics that have garnered much attention as precision biotherapeutics for their efficient hybridization properties and their exceptional biological and chemical stability. However, the poor cellular uptake of PNA is a limiting factor to its more extensive use in biomedicine; encapsulation in nanoparticle carriers has therefore emerged as a strategy for internalization and delivery of PNA in cells. In this study, we demonstrate that PNA can be readily loaded into porous silicon nanoparticles (pSiNPs) following a simple salt-based trapping procedure thus far employed only for negatively charged synthetic oligonucleotides. We show that the ease and versatility of PNA chemistry also allows for producing PNAs with different net charge, from positive to negative, and that the use of differently charged PNAs enables optimization of loading into pSiNPs. Differently charged PNA payloads determine different release kinetics and allow modulation of the temporal profile of the delivery process.iIn vitro/isilencing of a set of specific microRNAs using a pSiNP-PNA delivery platform demonstrates the potential for biomedical applications. |
| Databáze: | OpenAIRE |
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