Dysregulation in the expression of (lncRNA-TSIX, TP53INP2 mRNA, miRNA-1283) in spinal cord injury
| Autor: | Mohamed Farid El-Asmer, Marwa Matboli, Hanaa El-Tayeb Nasser, Ibrahim Abdelmohsen Abdelnaiem, Ayman El-Sayed Shafei, Sara Mohammed Mohammed Salah |
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| Rok vydání: | 2020 |
| Předmět: |
Adult
Male 0106 biological sciences medicine.medical_specialty Pilot Projects Biology 01 natural sciences law.invention 03 medical and health sciences Downregulation and upregulation law Internal medicine microRNA Genetics medicine Humans RNA Messenger Inverse correlation Spinal cord injury Spinal Cord Injuries Polymerase chain reaction 030304 developmental biology 0303 health sciences Messenger RNA Nuclear Proteins Middle Aged medicine.disease Spinal cord MicroRNAs medicine.anatomical_structure Endocrinology Gene Expression Regulation Female RNA Long Noncoding Tsix 010606 plant biology & botany |
| Zdroj: | Genomics. 112:3315-3321 |
| ISSN: | 0888-7543 |
| DOI: | 10.1016/j.ygeno.2020.06.018 |
| Popis: | Aim The objective of this study is to examine the alterations in the levels of expression of serum lncRNA-TSIX, TP53INP2 mRNA, miRNA-1283 in spinal cord injured (SCI) patients versus healthy control. Method The expression of the selected RNAs in the sera was determined in 23 patients suffering from acute spinal cord injury, 41 individuals with chronic spinal cord injury, and 36 healthy control using real-time reverse-transcription polymerase chain reaction method. Results The results showed that lncRNA-TSIX and the TP53INP2 mRNA expression levels in SCI patients was overexpressed in comparison to the control group alongside with a significant downregulation of miR-1283. Statistically,there was a highly significant positive correlation between lnc-RNA-TRIX and TP53INP2 mRNA with inverse correlation between miRNA-1283 and lnc-RNA-TRIX based on fold changes. Conclusion Up-regulation of lncRNA-TSIX, TP53INP2 mRNA with downregulation of miRNA-1283 might be closely associated with progression of SCI. |
| Databáze: | OpenAIRE |
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