Anti-idiotypic antibodies mimic bovine viral diarrhea virus antigen
| Autor: | Maureen A. Rider, Frank Blecha, O. Y. AbdelMagid, H.C. Minocha, W. Xue, D.J. Orten |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
medicine.drug_class
viruses Enzyme-Linked Immunosorbent Assay Monoclonal antibody Binding Competitive Microbiology Virus Epitopes Virus antigen Antigen Antibody Specificity medicine Animals Antigens Viral Diarrhea Viruses Bovine Viral General Veterinary biology Virus receptor Pestivirus Antibodies Monoclonal General Medicine biology.organism_classification Precipitin Tests Virology Molecular biology Antibodies Anti-Idiotypic Polyclonal antibodies biology.protein Cattle Antibody |
| Zdroj: | Veterinary Microbiology. 29:201-212 |
| ISSN: | 0378-1135 |
| Popis: | Polyclonal rabbit anti-idiotypic antibodies (anti-ids) against two neutralizing murine monoclonal antibodies (mAbs) specific to a bovine viral diarrhea virus (BVDV) glycoprotein. 53 kDa, were produced, purified, and characterized. Each anti-id inhibited the binding of its respective mAb to BVDV antigen in a competitive ELISA and blocked the immunoprecipitation of the 53 kDa protein by the mAb. The anti-ids also inhibited the virus-neutralizing activity of their homologous mAbs. These results suggest that the anti-ids bear an internal image of a BVDV antigen and mimic neutralizing epitopes on the 53 kDa protein. Treatment of MDBK cells with the anti-ids inhibited BVDV infection, indicating that they block a cellular component, such as a virus receptor, required for virus adsorption or entry. Inhibition of the homologous mAb and lack of inhibition of the heterologous mAb indicate that the anti-ids are specific for the unique antigen-binding sites on the mAbs. |
| Databáze: | OpenAIRE |
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