The direct regulation of Aalbdsx on AalVgR is indispensable for ovarian development in Aedes albopictus
| Autor: | Binbin Jin, Jinbao Gu, Yan Sun, Peiwen Liu, Yijie Zhao, Xiaocong Li, Xin Zhang, Xiao-Guang Chen |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Male Sex Differentiation Doublesex Mosquito Vectors Biology 01 natural sciences Vitellogenin Vitellogenins RNA interference Aedes Gene expression Animals Gene Gene knockdown Sexual differentiation fungi General Medicine Cell biology 010602 entomology Insect Science Somatic sex determination biology.protein Female Agronomy and Crop Science Chromatin immunoprecipitation 010606 plant biology & botany |
| Zdroj: | Pest management scienceREFERENCES. 77(4) |
| ISSN: | 1526-4998 |
| Popis: | BACKGROUNDAedes albopictusis an important vector with an extensive worldwide distribution. Only female mosquitoes play a significant role in the transmission of pathogens.Doublesex(dsx) is a central nexus gene in the insect somatic sex determination hierarchy.RESULTSIn this study, we characterized the full-length sex-specific splicing forms of theAe. albopictus dsxgene (Aalbdsx). Then, we identified 15 direct target genes of DSX in adult females using digital gene expression (DGE) combined with qPCR following a chromatin immunoprecipitation assay (ChIP) with specific DSX antibodies. The knockdown ofAalbdsxsuppressed ovarian development, and the transcript levels of theAalbdsxtargetvitellogenin receptor(VgR) gene decreased, whereasvitellogenin(Vg) expression showed an increase in the fat body. Genes in the majorVgregulatory pathway were also upregulated. Our results suggest that the effects ofAalbdsxRNAi on ovarian development are exerted mainly viaVgRrather thanVg.CONCLUSIONThe results of our study not only provide a reference for the further elucidation of the sex determination cascade and comparative analyses ofdsxtarget interactions in mosquitoes but also reveal potential molecular targets for application to the development of sterile male mosquitoes to be released for vector control. |
| Databáze: | OpenAIRE |
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