Evaluation of the monocyte counting by two automated haematology analysers compared with flow cytometry
| Autor: | Ernesto Grimaldi, P. Carandente, M. F. Romano, M. De Caterina, R. Bisogni, F. Scopacasa, M. Pellegrino |
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| Přispěvatelé: | Grimaldi, Ernesto, CARANDENTE GIARRUSSO, Patrizia, Scopacasa, FRANCESCO UMBERTO VITTOR, Romano, MARIA FIAMMETTA, Pellegrino, Maria, Bisogni, Rita, DE CATERINA, Maurizio |
| Rok vydání: | 2005 |
| Předmět: |
Pathology
medicine.medical_specialty Clinical Biochemistry Statistical difference Monocytes Mean difference Flow cytometry Automation Leukocyte Count Deming regression Monocyte count medicine Humans Blood Cells Hematologic Tests Chromatography medicine.diagnostic_test business.industry Monocyte Biochemistry (medical) Becton dickinson Reproducibility of Results Hematology General Medicine Flow Cytometry hematology analyser medicine.anatomical_structure count monocyte Tukey's range test business |
| Zdroj: | Clinical and Laboratory Haematology. 27:91-97 |
| ISSN: | 1365-2257 0141-9854 |
| DOI: | 10.1111/j.1365-2257.2005.00676.x |
| Popis: | Summary The aim is to determine the monocyte count performance of the Bayer Diagnostics ADVIA®120 and Coulter® LH 750 automated haematology analysers and the results obtained by these two instruments were compared with those provided by Becton Dickinson FACScan flow cytometer using the combination of CD45/CD14 MoAb. Linearity and imprecision were also evaluated. The linearity of both instruments was good. Coulter® LH 750 showed better precision (4.3%) than ADVIA 120 (9.0%) both within and between batch. A significant correlation (r = 0.973) was found between the LH 750 and the flow cytometry method, while a modest one was observed between the latter and the ADVIA 120 (r = 0.880). When comparing the percentage of monocytes by means of one-way anova and Tukey test, it was found that the LH 750 provided the closest results in comparison with flow cytometry, with no statistical difference between the means (mean difference MO% = 0.6); however the difference was statistically different between the ADVIA 120 and flow cytometry (mean difference MO% = −4.06). These data were confirmed by Altman–Bland and Deming regression analyses. |
| Databáze: | OpenAIRE |
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