Rapid determination of propyphenazone in plasma by high-performance liquid chromatography
| Autor: | J. Campestrini, J. Godbillon, M.C. Rouan, J.B. Lecaillon |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Detection limit
Chromatography Ethanol Chemistry Potassium Anti-Inflammatory Agents Non-Steroidal chemistry.chemical_element Reproducibility of Results General Chemistry High-performance liquid chromatography Blood proteins chemistry.chemical_compound Phenylbutazone medicine Humans Spectrophotometry Ultraviolet Acetonitrile Propyphenazone Antipyrine Chromatography High Pressure Liquid medicine.drug |
| Zdroj: | Journal of chromatography. 577(2) |
| Popis: | A rapid and simple high-performance liquid chromatographic assay for the determination of propyphenazone in plasma is described. Phenylbutazone was used as the internal standard. Plasma proteins were precipitated with acetonitrile before injection onto a 3-μm Supelcosil LC-18 column. The mobile phase, ethanol containing 0.2% (v/v) heptylamine—0.005 M potassium dihydrogenphosphate (30:70, v/v), was used at a flow-rate of 1.3 ml/min. The quantitation was performed by ultraviolet detection at a wavelength of 270 nm. The chromatographic time was 7 min. The within- and between-day coefficients of variation were less than 6% and the recoveries close to 100% for concentrations between 0.4 and 22 μmol/l. The limit of quantitation was 0.4 μmol/l ( ca. 100 ng/ml). |
| Databáze: | OpenAIRE |
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