Recruitment of a Neuronal Ensemble in the Central Nucleus of the Amygdala Is Required for Alcohol Dependence
| Autor: | Olivier George, Giordano de Guglielmo, Leandro F. Vendruscolo, Elena Crawford, Molly Brennan, Maury Cole, Bruce T. Hope, George F. Koob, Sarah Kim |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Time Factors Nerve net Self Administration Alcohol Cardiovascular Medical and Health Sciences Transgenic Oral and gastrointestinal Substance Misuse Alcohol Use and Health chemistry.chemical_compound 0302 clinical medicine Cancer media_common Neurons alcohol General Neuroscience Central nucleus of the amygdala Statistics Articles Daun02 Stroke Alcoholism medicine.anatomical_structure Anesthesia addiction Rats Transgenic Self-administration Psychology medicine.medical_specialty Reinforcement Schedule media_common.quotation_subject Statistics Nonparametric Operant 03 medical and health sciences Internal medicine medicine Animals Nonparametric Neurology & Neurosurgery Ethanol Animal neuronal ensembles Prevention Addiction Psychology and Cognitive Sciences Central Amygdaloid Nucleus Daunorubicin Alcohol dependence Neurosciences Central Nervous System Depressants dependence Abstinence Brain Disorders Rats Disease Models Animal CeA Good Health and Well Being Oncogene Proteins v-fos 030104 developmental biology Endocrinology chemistry Disease Models Conditioning Operant Nerve Net 030217 neurology & neurosurgery Conditioning |
| Zdroj: | The Journal of neuroscience : the official journal of the Society for Neuroscience, vol 36, iss 36 |
| ISSN: | 1529-2401 0270-6474 |
| Popis: | Abstinence from alcohol is associated with the recruitment of neurons in the central nucleus of the amygdala (CeA) in nondependent rats that binge drink alcohol and in alcohol-dependent rats. However, whether the recruitment of this neuronal ensemble in the CeA is causally related to excessive alcohol drinking or if it represents a consequence of excessive drinking remains unknown. We tested the hypothesis that the recruitment of a neuronal ensemble in the CeA during abstinence is required for excessive alcohol drinking in nondependent rats that binge drink alcohol and in alcohol-dependent rats. We found that inactivation of the CeA neuronal ensemble during abstinence significantly decreased alcohol drinking in both groups. In nondependent rats, the decrease in alcohol intake was transient and returned to normal the day after the injection. In dependent rats, inactivation of the neuronal ensemble with Daun02 produced a long-term decrease in alcohol drinking. Moreover, we observed a significant reduction of somatic withdrawal signs in dependent animals that were injected with Daun02 in the CeA. These results indicate that the recruitment of a neuronal ensemble in the CeA during abstinence from alcohol is causally related to excessive alcohol drinking in alcohol-dependent rats, whereas a similar neuronal ensemble only partially contributed to alcohol-binge-like drinking in nondependent rats. These results identify a critical neurobiological mechanism that may be required for the transition to alcohol dependence, suggesting that focusing on the neuronal ensemble in the CeA may lead to a better understanding of the etiology of alcohol use disorders and improve medication development.SIGNIFICANCE STATEMENTAlcohol dependence recruits neurons in the central nucleus of the amygdala (CeA). Here, we found that inactivation of a specific dependence-induced neuronal ensemble in the CeA reversed excessive alcohol drinking and somatic signs of alcohol dependence in rats. These results identify a critical neurobiological mechanism that is required for alcohol dependence, suggesting that targeting dependence neuronal ensembles may lead to a better understanding of the etiology of alcohol use disorders, with implications for diagnosis, prevention, and treatment. |
| Databáze: | OpenAIRE |
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