Time-Course Changes of Extracellular Matrix Encoding Genes Expression Level in the Spinal Cord Following Contusion Injury—A Data-Driven Approach

Autor: Luca Lorenzini, Zahra Khalajzeyqami, Andrea Bighinati, Maura Cescatti, Marzia Moretti, Laura Calzà, Vito Antonio Baldassarro, Luciana Giardino
Přispěvatelé: Bighinati A., Khalajzeyqami Z., Baldassarro V.A., Lorenzini L., Cescatti M., Moretti M., Giardino L., Calza Laura
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0301 basic medicine
Time Factors
secondary degeneration
Extracellular matrix
lcsh:Chemistry
0302 clinical medicine
Cell Movement
Gene expression
Spinal cord injury
lcsh:QH301-705.5
Spectroscopy
Cells
Cultured
TIMP1
Spinal Cord Injurie
Cell migration
General Medicine
Contusion
Computer Science Applications
Cell biology
medicine.anatomical_structure
Hyaluronan Receptors
Spinal Cord
Female
medicine.symptom
Astrocyte
Human
Time Factor
Contusions
extracellular matrix
Primary Cell Culture
Biology
Catalysis
Article
Inorganic Chemistry
Lesion
03 medical and health sciences
medicine
Cell Adhesion
Animals
Humans
Physical and Theoretical Chemistry
Molecular Biology
Spinal Cord Injuries
Tissue Inhibitor of Metalloproteinase-1
Animal
Gene Expression Profiling
Organic Chemistry
CD44
Computational Biology
Spinal cord
medicine.disease
Hyaluronan Receptor
spinal cord injury
Rats
Disease Models
Animal
030104 developmental biology
remyelination
timp1
Gene Expression Regulation
lcsh:Biology (General)
lcsh:QD1-999
inflammation
Astrocytes
biology.protein
Rat
030217 neurology & neurosurgery
Zdroj: International Journal of Molecular Sciences, Vol 22, Iss 1744, p 1744 (2021)
International Journal of Molecular Sciences
Volume 22
Issue 4
ISSN: 1661-6596
1422-0067
Popis: The involvement of the extracellular matrix (ECM) in lesion evolution and functional outcome is well recognized in spinal cord injury. Most attention has been dedicated to the “core” area of the lesion and scar formation, while only scattered reports consider ECM modification based on the temporal evolution and the segments adjacent to the lesion. In this study, we investigated the expression profile of 100 genes encoding for ECM proteins at 1, 8 and 45 days post-injury, in the spinal cord segments rostral and caudal to the lesion and in the scar segment, in a rat model. During both the active lesion phases and the lesion stabilization, we observed an asymmetric gene expression induced by the injury, with a higher regulation in the rostral segment of genes involved in ECM remodeling, adhesion and cell migration. Using bioinformatic approaches, the metalloproteases inhibitor Timp1 and the hyaluronan receptor Cd44 emerged as the hub genes at all post-lesion times. Results from the bioinformatic gene expression analysis were then confirmed at protein level by tissue analysis and by cell culture using primary astrocytes. These results indicated that ECM regulation also takes place outside of the lesion area in spinal cord injury.
Databáze: OpenAIRE
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